Eleven novel microsatellite loci for Japanese whiting (Sillago japonica) and cross amplification in the endangered small-scale sillago (Sillago parvisquamis)

• Published in: Conservation genetics resources Vol. 5; no. 3; pp. 659 - 662
• Main Authors: Ueno, Kanako, Watanabe, Makoto, Ahmad-Syazni, Kamarudin, Koike, Masaru, Ohara, Kenichi, Umino, Tetsuya
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2013; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Conservation Biology/Ecology; Ecology; Evolutionary Biology; Fisheries; Heterozygosity; Life Sciences; Linkage disequilibrium; Microsatellites; Plant Genetics and Genomics; Population structure; Sillago japonica; Sillago parvisquamis; Species; Technical Note; Japan; Genomic library; Microsatellite; Conservation genetics
• ISSN: 1877-7252; 1877-7260
• DOI: 10.1007/s12686-013-9876-x

Japanese whiting ( Sillago japonica ) are a relatively common species that inhabit coastal shallow waters in Japan and are the target species in an important recreational fishery. We isolated eleven candidate microsatellite loci from a small insert genomic DNA library of S. japonica. We screened for polymorphisms in the eleven loci using wild individuals (n = 48) collected from Suounada Sound, in the Seto Inland Sea, Japan. The number of alleles per locus ranged from 6 to 26 with no evidence of linkage disequilibrium. Observed heterozygosity ranged from 0.58 to 0.98 with one locus exhibiting a significant departure from Hardy–Weinberg equilibrium. A test for cross-amplification using the closely related species, Sillago parvisquamis yielded scoreable peaks and a high level of polymorphism in four loci. These polymorphic microsatellites can be used to identify population structure in S. japonica and provide potential markers for the endangered S. parvisquamis .