Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)

The Chinese concave-eared frog is a rare species endemic to a restricted region of easternChina. In the present study, we isolated twelve polymorphic microsatellite loci from a dinucleotide—or trinucleotide—enriched library for the frog. The number of alleles per locus ranged from 2 to 11 with an av...

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Published inConservation genetics resources Vol. 3; no. 2; pp. 225 - 227
Main Authors Yan, Ji-Chun, Luo, Tao-Lin, Wu, Hai-Long
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.04.2011
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Endemic species
Evolutionary Biology
Gene flow
Genetic structure
Life Sciences
Plant Genetics and Genomics
Rare species
Technical Note
Odorrana tormota
Polymorphic microsatellite loci
Chinese concave-eared frog
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Summary:The Chinese concave-eared frog is a rare species endemic to a restricted region of easternChina. In the present study, we isolated twelve polymorphic microsatellite loci from a dinucleotide—or trinucleotide—enriched library for the frog. The number of alleles per locus ranged from 2 to 11 with an average of 5.92. The observed and expected heterozygosities varied from 0.063 to 0.862 and from 0.062 to 0.873, with an average of 0.453 and 0.601, respectively. The polymorphic microsatellite locidescribed here are expected to be useful for further studies of the genetic structure, gene flow andmating mechanism in Rana tormotus, which in turn will be helpful in developing conservation andmanagement strategies for the rare frog.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-010-9328-9