Synthetic Antimicrobial Peptides. II. Antimicrobial and Hemolytic Activity of Cationic Peptides Containing Cysteine Residues with Free Sulfhydryl Groups
— The antimicrobial and hemolytic activities of R 9 F 2 С 2 ( P1ss ), (KFF) 3 KС 2 ( P2ss ), and (RAhaR) 4 AhaβAС 2 ( P3ss ) (where Aha is 6-aminohexanoic acid and βA is beta-alanine) synthetic antimicrobial peptides (SAMP) with different amphipathic properties and containing the cysteine residues w...
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Published in | Russian journal of bioorganic chemistry Vol. 45; no. 6; pp. 833 - 841 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.11.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | —
The antimicrobial and hemolytic activities of R
9
F
2
С
2
(
P1ss
), (KFF)
3
KС
2
(
P2ss
), and (RAhaR)
4
AhaβAС
2
(
P3ss
) (where Aha is 6-aminohexanoic acid and βA is beta-alanine) synthetic antimicrobial peptides (SAMP) with different amphipathic properties and containing the cysteine residues with free sulfhydryl groups were studied. The introduction of cysteine residues into the composition of SAMP was shown to increase their antimicrobial activity 3–7 times, while their hemolytic activity increased 2–12 times in relation to human erythrocytes for different peptides in different ways, which determined their different selectivity. The
P1ss
peptide with a linear type of amphipathicity showed the highest antimicrobial activity and high selectivity against the fungus
Candida albicans
and bacterium
Staphylococcus aureus
(MIC 0.5 μM; TI 52 μM). The
P1ss
peptide possessed not only greater antimicrobial activity against pathogenic fungus
C. albicans
in comparison to the
P2ss
peptide (MIC 3.9 μM) with the classical helical amphipathicity, but also more than three times lower hemolytic activity (MHC 26 and 8 μM, respectively). Therefore, TI for the
P2ss
peptide (TI 2.1) turned out to be more than 20 times lower than that for the
P1ss
peptide. Thus, the
P1ss
peptide is the most promising antimicrobial preparation among the studied SAMP. |
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ISSN: | 1068-1620 1608-330X |
DOI: | 10.1134/S1068162019060037 |