Isolation and characterization of 18 microsatellites for Lycengraulis grossidens (Pisces: Clupeiformes)

Twelve polymorphic and six non-polymorphic microsatellite loci were isolated and characterized from a CT–GT enriched genomic library of Lycengraulis grossidens . The number of alleles ranged from 1 to 29. The observed and expected heterozygosity ranged from 0.19 to 0.8 and from 0.18 to 0.95, respect...

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Published inConservation genetics resources Vol. 5; no. 1; pp. 15 - 18
Main Authors Mai, Ana C. G., Vieira, João P., Plavan, Alicia A., Nobrega, Marcelo F., Moraes, Leonardo E., Rodrigues, Fábio L., Marins, Luis F.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.03.2013
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Food sources
Gene flow
Genetic diversity
Heterozygosity
Life Sciences
Lycengraulis grossidens
Microsatellites
Plant Genetics and Genomics
Population genetics
Population studies
Technical Note
Engraulididae
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Summary:Twelve polymorphic and six non-polymorphic microsatellite loci were isolated and characterized from a CT–GT enriched genomic library of Lycengraulis grossidens . The number of alleles ranged from 1 to 29. The observed and expected heterozygosity ranged from 0.19 to 0.8 and from 0.18 to 0.95, respectively. Cross-species amplification of the markers developed for L. grossidens was successful in the wild Engraulididae species Anchoa spinifer , Anchoa marinii, Anchoviella lepidentostole , Cetengraulis edentulous and Engraulis anchoita . The developed microsatellite loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow for these important food sources.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-012-9718-2