Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

Methodological studies are an important previous step in molecular research. We optimize a method for DNA extraction from eggs and a noninvasive extraction method for adult tissues of Maja brachydactyla. Our results show that lyophilized eggs in B developmental stage are the most suitable for DNA ex...

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Bibliographic Details
Published inConservation genetics resources Vol. 9; no. 3; pp. 369 - 370
Main Authors Rodríguez-Pena, Elba, Verísimo, Patricia, Fernández, Luis, González-Tizón, Ana, Martínez-Lage, Andrés
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.09.2017
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Deoxyribonucleic acid
DNA
Ecology
Eggs
Endangered species
Evolutionary Biology
Life Sciences
Plant Genetics and Genomics
Setae
Technical Note
Crab
Sample conservation
Sampling without death
Egg developmental stage
DNA extraction method
Maja
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Summary:Methodological studies are an important previous step in molecular research. We optimize a method for DNA extraction from eggs and a noninvasive extraction method for adult tissues of Maja brachydactyla. Our results show that lyophilized eggs in B developmental stage are the most suitable for DNA extraction. Regarding adult tissues, we propose setae as an alternative tissue for DNA extraction, since prevents mutilation of specimens, which avoids infections and fitness reduction. That is especially relevant in studies with endangered species of crustaceans.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-017-0685-5