Mevalonate pathway genes expressed in chilli CM334 inoculated with Phytophthora capsici and infected by Nacobbus aberrans and Meloidogyne enterolobii
The chilli pepper landrace CM334 ( Capsicum annuum L.) is resistant to Phytophthora capsici ( Pc ), but susceptible to Meloidogyne enterolobii ( Me ) and Nacobbus aberrans ( Na ) . Resistance to Pc is associated with capsidiol (sesquiterpene phytoalexin). To know the transcriptional alterations that...
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Published in | European journal of plant pathology Vol. 148; no. 4; pp. 867 - 881 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.08.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The chilli pepper landrace CM334 (
Capsicum annuum
L.) is resistant to
Phytophthora capsici
(
Pc
), but susceptible to
Meloidogyne enterolobii
(
Me
) and
Nacobbus aberrans
(
Na
)
.
Resistance to
Pc
is associated with capsidiol (sesquiterpene phytoalexin). To know the transcriptional alterations that
Na
or
Me
induce in CM334 plants, the expression levels of some genes of the mevalonate pathway were determined by RT-qPCR. At 3 or 21 days after inoculation with nematode (dai), plant stems were inoculated with
Pc
(
NaPc
and
MePc
treatments)
,
furthermore, there were also plants inoculated only with
Pc, Na
, and
Me
. At 6, 24 and 48 h after inoculation with
Pc
(haio), the transcripts accumulation of
HMG2
(hydroxymethylglutaryl-CoA reductase 2),
EAS
(5-epiaristolochene synthase) (associated with sesquiterpene phytoalexin biosynthesis),
HMG3
and
SS
genes (squalene synthase) was assessed; also capsidiol accumulation was evaluated at 144 haio. The transcript levels were generally lower in
NaPc, MePc
,
Na
and
Me
than those recorded in the
Pc
treatment (
p˂
0.05) at 3 and 21 dai with
Na
or
Me
. Only when
Pc
was inoculated at 21 dai with the nematode, the capsidiol levels were significantly lower in stems of plants infected by
NaPc
and
MePc
compared to
Pc
treatment. In contrast, in roots the differences between
Pc
vs
NaPc
and
Pc
vs
MePc
, were significant both at 3 and 21 dai (
p˂
0.05). The two nematodes altered the expression of defense genes systemically (stems), and reduced capsidiol levels locally and systemically, possibly to create a favorable environment that allowed them to complete their life cycle. |
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ISSN: | 0929-1873 1573-8469 |
DOI: | 10.1007/s10658-016-1142-0 |