Human T lymphocytes require lipid as either lipoprotein or nonesterified fatty acid for in vitro activation

Serum, which is required for activation of T cells in vitro, was fractionated to isolate and identify major supportive components. The serum activity concentrated exclusively with the lipoprotein fractions LDL and HDL. Furthermore, oleic and linoleic acids were equally active supplements, suggesting...

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Bibliographic Details
Published inImmunological investigations Vol. 20; no. 5-6; p. 507
Main Author Herzberg, V L
Format Journal Article
LanguageEnglish
Published England 1991
Subjects
Adult
Antigens, Differentiation, T-Lymphocyte - immunology
Fatty Acids, Nonesterified - immunology
Fatty Acids, Nonesterified - metabolism
Growth Substances - blood
Humans
Linoleic Acids - metabolism
Lipoproteins - immunology
Lipoproteins - isolation & purification
Lipoproteins - metabolism
Lipoproteins, HDL - metabolism
Lipoproteins, LDL - metabolism
Lymphocyte Activation
Oleic Acids - metabolism
T-Lymphocytes - immunology
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Summary:Serum, which is required for activation of T cells in vitro, was fractionated to isolate and identify major supportive components. The serum activity concentrated exclusively with the lipoprotein fractions LDL and HDL. Furthermore, oleic and linoleic acids were equally active supplements, suggesting that resting T cells may require exogenous lipid because they are incapable of synthesizing fatty acid. A survey of fatty acids disclosed that all were capable of some degree of support. Titration delineated the narrow concentration range of lipid supplements successful in supporting T cell proliferation and demonstrated that concentrations must be rigidly controlled to optimize cell proliferation.
ISSN:0882-0139
DOI:10.3109/08820139109082631