Dynamics of activity of intracellular cysteine-dependent proteases and some peptidases in embryogenesis of Atlantic salmon Salmo salar L

• Published in: Russian journal of developmental biology Vol. 48; no. 4; pp. 263 - 268
• Main Authors: Nemova, N. N., Kyaivyaryainen, E. I., Krupnova, M. Yu
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2017; Springer Nature B.V
• Subjects: Animal Anatomy; Biomedical and Life Sciences; Blastula; Calcium; Calpain; Cathepsin B; Cysteine; Developmental Biology; Eggs; Embryogenesis; Embryogenesis and Carcinogenesis; Enzymes; Eye; Fertilization; Hatching; Histology; Intracellular; Life Sciences; Morphology; Pigmentation; Proteases; Protein turnover; Proteolysis; Proteolytic enzymes; Salmo salar; Salmon; proteolytic enzymes; freshwater salmon; embryogenesis
• ISSN: 1062-3604; 1608-3326
• DOI: 10.1134/S1062360417040075

The dynamics of the activity of cysteine-dependent enzymes involved in intracellular proteolysis was studied in freshwater salmon Salmo salar L. during its early development: in the period from the blastodisc formation to hatching and in eggs before fertilization. The Ca 2+ -activated proteolytic activity of calpains, the activity of acidic lysosomal protease cathepsin B, and the activity of several peptidases that use the chromogenic component p -nitroaniline-containing peptides (L-Ala-pNA, L-Pro-pNA, L-Arg-pNA, N-Bzl-L-Arg-pNA, L-Phe-pNA, N-Glu-L-Phe-pNA, and Z-Gly-Pro-pNA) as substrates were analyzed. It is shown that the activity of the enzymes of interest increases at the stage of late blastula and early gastrula. The next increase in the activity of cathepsin B and calpain is observed at the stage of eye pigmentation, and the activity of peptidases and cathepsin B increases in eggs before hatching. The functional role of changes in intracellular proteolytic enzymes in the processes of protein metabolism associated with the Atlantic salmon embryo is discussed.