Development of barcode and proteome profiling of glioblastoma

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 8; no. 3; pp. 243 - 251
• Main Authors: Naryzhny, S. N., Ronzhina, N. L., Mainskova, M. A., Belyakova, N. V., Pantina, R. A., Filatov, M. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2014; Springer Nature B.V
• Subjects: Bioorganic Chemistry; Brain cancer; Chemistry; Chemistry and Materials Science; Immunohistochemistry; Medicinal Chemistry; Proteins; Proteomics; Tumors; glioblastoma; PCNA; proteomics; p53
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750814030111

High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments, we used several glioblastoma cell lines to obtain proteomics information specific for this disease. 2DE separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us to detecting more than 600 protein spots and identifying more than 130 of them. Proteome profiles in normal and glioblastoma cell lines are very similar but levels of several proteins have prominent differences between norm and cancer. Among these proteins are alpha-enolase (ENOA_HUMAN), pyruvate kinase M1/M2 (KPYM_HUMAN), cofilin 1 (COF1_HUMAN), translationally-controlled tumor protein TCTP_HUMAN, annexin 1 (ANXA1_HUMAN), PCNA (PCNA_HUMAN), p53 (TP53_HUMAN) and others. Most interesting results were obtained about protein p53. Its level was dramatically up-regulated and enriched by multiple additional isoforms in all glioblastoma cell lines. An immunological analysis (Western blot) of three hub-proteins (p53, 14-3-3, PCNA) allowed us to creating the minimal barcode of glioblastoma cell lines. These preliminary data point to this barcode as a promising diagnostic tool for testing of the biological fluids from patients.