Construction and Characterization of a Recombinant Mutant Homolog of the CheW Protein from Thermotoga petrophila RKU-1

In this work, a recombinant chemotaxis CheW protein from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homologue (TpeCheW-mut) have been obtained. Despite the low homology with the CheW protein of the Escherichia coli bacteria, these proteins do not cause metabolic overload and are well expre...

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Published inBiochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 12; no. 2; pp. 143 - 150
Main Authors Grishin, D. V., Zhdanov, D. D., Gladilina, Ju. A., Pokrovsky, V. S., Podobed, O. V., Pokrovskaya, M. V., Aleksandrova, S. S., Milyushkina, A. L., Vigovskiy, M. A., Sokolov, N. N.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.04.2018
Springer Nature B.V
Subjects
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Chemotaxis
CheW protein
Homology
Medicinal Chemistry
Proteins
Thermotoga petrophila
biotechnology
recombinant thermostable CheW protein
hyperthermophilic microorganism
protein overexpression
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Summary:In this work, a recombinant chemotaxis CheW protein from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homologue (TpeCheW-mut) have been obtained. Despite the low homology with the CheW protein of the Escherichia coli bacteria, these proteins do not cause metabolic overload and are well expressed by E. coli laboratory strains. A wide range of features and parameters important for isolation of the TpeCheW-mut protein, such as stability over a wide range of temperatures and pH values, high level of expression, solubility, and the possibility of using simple low-stage purification schemes, including heat pretreatment, has been recognized. Possible directions of using this protein in the practice of scientific and applied research have been formulated and justified.
ISSN:1990-7508
1990-7516
DOI:10.1134/S1990750818020051