A New Iq-Peptide of the Kunitz Type from the Heteractis magnifica Sea Anemone Exhibits Neuroprotective Activity in a Model of Alzheimer’s Disease

The HMIQ3c1 recombinant peptide (6434 Da), the Kunitz-type protease inhibitor, which belonged to the new group of the IQ-peptides from the Heteractis magnifica sea anemone was prepared. The inhibitory constant of trypsin by this peptide proved to be 5.0 × 10 –8 M. rHMIQ3c1 was shown to have no inter...

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Published inRussian journal of bioorganic chemistry Vol. 44; no. 4; pp. 416 - 423
Main Authors Kvetkina, A. N., Leychenko, E. V., Yurchenko, E. A., Pislyagin, E. A., Peigneur, S., Tytgat, Y., Isaeva, M. P., Aminin, D. L., Kozlovskaya, E. P.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.07.2018
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Life Sciences
Organic Chemistry
Peptides
Protease inhibitors
Trypsin
Kunitz-type protease inhibitors
sea anemone
neuroprotective activity
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Summary:The HMIQ3c1 recombinant peptide (6434 Da), the Kunitz-type protease inhibitor, which belonged to the new group of the IQ-peptides from the Heteractis magnifica sea anemone was prepared. The inhibitory constant of trypsin by this peptide proved to be 5.0 × 10 –8 M. rHMIQ3c1 was shown to have no interaction with eight isoforms of Kv1 channels. This peptide exhibited neuroprotective activity in a concentration of 10 μM, and the peptide increased the viability of cells of the Neuro2a murine neuroblastoma by 39.4 ± 6.6% in the presence of β-amyloid. However, the peptide did not influence the viability of the cells during their incubation with 6-hydroxydofamine.
ISSN:1068-1620
1608-330X
DOI:10.1134/S106816201804012X