Electrode assemblies composed of redox cascades from microbial respiratory electron transfer chains

Respiratory and photosynthetic electron transfer chains are dependent on vectorial electron transfer through a series of redox proteins. Examples include electron transfer from NapC to NapAB nitrate reductase in Paracoccus denitrificans and from CymA to Fcc3 (flavocytochrome c3) fumarate reductase i...

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Published inBiochemical Society transactions Vol. 41; no. 5; p. 1249
Main Authors Gates, Andrew J, Marritt, Sophie J, Bradley, Justin M, Shi, Liang, McMillan, Duncan G G, Jeuken, Lars J C, Richardson, David J, Butt, Julea N
Format Journal Article
LanguageEnglish
Published England 01.10.2013
Subjects
Cell Respiration - genetics
Cytochrome c Group - chemistry
Electron Transport - genetics
Nitrate Reductase - chemistry
Nitrate Reductases - chemistry
Oxidation-Reduction
Paracoccus denitrificans - enzymology
Photosynthesis - genetics
Shewanella - enzymology
Succinate Dehydrogenase - chemistry
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Summary:Respiratory and photosynthetic electron transfer chains are dependent on vectorial electron transfer through a series of redox proteins. Examples include electron transfer from NapC to NapAB nitrate reductase in Paracoccus denitrificans and from CymA to Fcc3 (flavocytochrome c3) fumarate reductase in Shewanella oneidensis MR-1. In the present article, we demonstrate that graphite electrodes can serve as surfaces for the stepwise adsorption of NapC and NapAB, and the stepwise adsorption of CymA and Fcc3. Aspects of the catalytic properties of these assemblies are different from those of NapAB and Fcc3 adsorbed in isolation. We propose that this is due to the formation of NapC-NapAB and of CymA-Fcc3 complexes that are capable of supporting vectorial electron transfer.
ISSN:1470-8752
DOI:10.1042/BST20130147