Carrageenanolytic enzymes from marine bacteria associated with the red alga Tichocarpus crinitus
One hundred six bacterial strains (106 strains) were isolated from the Pacific red alga Tichocarpus crinitus , collected from the Troitsa Bay of Gulf of Peter the Great (Sea of Japan, Russia), and studied on their ability to degrade different samples of carrageenan as substrates. Some of the studied...
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Published in | Journal of applied phycology Vol. 30; no. 3; pp. 2071 - 2081 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | One hundred six bacterial strains (106 strains) were isolated from the Pacific red alga
Tichocarpus crinitus
, collected from the Troitsa Bay of Gulf of Peter the Great (Sea of Japan, Russia), and studied on their ability to degrade different samples of carrageenan as substrates. Some of the studied strains exhibited hydrolytic activity selectively to a total polysaccharide (kappa and lambda) from
Chondrus armatus
, other strains, to κ/β-carrageenan from
T
.
crinitus
, which did not possess the same effect to a total polysaccharide from
T
.
crinitus
. The strains with the highest enzyme activity (28 strains) were identified using 16S rRNA gene sequence techniques and classified to the phyla
Bacteroidetes
and
Proteobacteria
. Most (75%) of the studied carrageenase producers belong to phylum
Bacteroidetes
(21 strains). These strains were related with 7 phylotypes of the genera
Aquimarina
,
Cellulophaga
,
Maribacter
, and
Zobellia
(family
Flavobacteriaceae
of the class
Flavobacteriia
).
Cellulophaga
strains were the dominant group (35.7% of total) and closely related to species
C
.
baltica
and
C
.
lytica
with 99.8 and 99.9% sequence similarity, respectively. The remaining strains (7 strains) were represented by members of the genera
Altererythrobacter
(the family
Erythrobacteraceae
),
Phaeobacter
,
Sulfitobacter
,
Tateyamaria
(the family
Rhodobacteraceae
), and
Sphingomonas
(the family
Sphingobacteriaceae
) of the class
Alphaproteobacteria
and
Psychrobacter
(the family
Moraxellaceae
) and
Vibrio
(the family
Vibrionaceae
) of the class
Gammaproteobacteria. |
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ISSN: | 0921-8971 1573-5176 |
DOI: | 10.1007/s10811-017-1355-4 |