The combined action of binase and bleomycin on human lung adenocarcinoma cells

Some microbial ribonucleases (RNases) demonstrate selective cytotoxic effect against a wide range of tumor cells. In this context combined use of cytotoxic RNases in complex therapy with other chemotherapeutic agents appears to be especially promising. In this study we have investigated the apoptosi...

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Published inBiochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 10; no. 1; pp. 87 - 90
Main Authors Zelenikhin, P. V., Makeeva, A. V., Nguen, T. N., Siraj, Y. A., Ilinskaya, O. N.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 2016
Springer Nature B.V
Subjects
Bioorganic Chemistry
Cellular biology
Chemistry
Chemistry and Materials Science
Cytotoxicity
Lung cancer
Medicinal Chemistry
Tumors
bleomycin
binase
antitumor activity
lung adenocarcinoma
cytotoxic ribonucleases
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Summary:Some microbial ribonucleases (RNases) demonstrate selective cytotoxic effect against a wide range of tumor cells. In this context combined use of cytotoxic RNases in complex therapy with other chemotherapeutic agents appears to be especially promising. In this study we have investigated the apoptosisinduced effect of Bacillus pumilus RNase (binase) in combination with known antitumor antibiotic bleomycin on human lung adenocarcinoma A549 cells. The combined effect of high concentrations of these agents did not have any mutual increase in their apoptosisinduced action, while a combination of nonapoptotic concentrations resulted in the increase of proportion of apoptotic cells up to 22% as compared with individual effect of bleomycin (6%) and binase (12%) used separately. These results indicate that binase and bleomycin are effective in combination of their low concentrations and ineffective in combination of their high concentrations.
ISSN:1990-7508
1990-7516
DOI:10.1134/S1990750816010121