Bactericidal Efficacy of New Types of Magnesium Hydroxide and Calcium Carbonate Nanoparticles

• Published in: Molecular genetics, microbiology and virology Vol. 34; no. 4; pp. 252 - 262
• Main Authors: Sanaa G. Al Attas, Faten Al-Hazmi, Alwafi, Reem, Alahmadi, Ahlam A., Al-Quwaie, Diana A.H., Bahieldin, Ahmed, Edris, Sherif
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.10.2019; Springer Nature B.V
• Subjects: Antibacterial activity; Bacteria; Biomedical and Life Sciences; Calcium carbonate; Calcium hydroxide; Gram-negative bacteria; Gram-positive bacteria; Growth rate; Life Sciences; Magnesium; Microbiology; Molecular Medicine; Nanoparticles; Silver
• ISSN: 0891-4168; 1934-841X
• DOI: 10.3103/S0891416819040086

Objectives of the present study were to evaluate the antibacterial activity of the two types of inorganic magnesium hydroxide [Mg(OH) 2 ] and calcium carbonate [CaCO 3 ] nanoparticles (NPs) on the growth of three Gram-negative bacteria, e.g., Escherichia coli , Pseudomonas aeruginosa and Serratia marcescens and three Gram-positive bacteria, e.g., Streptococcus pyogenes , Staphylococcus aureus and Streptococcus bovis. The synthesis of these NPs was done by a microwave hydrothermal method. The structures and sizes of synthesized nanoparticles were investigated using X-ray diffractometer. Antimicrobial susceptibility of different NPs was determined at 20, 50 and 100 mg/mL by the agar-well diffusion method, growth reduction at the aqueous solution and time-kill assay. The antimicrobial effects across NPs and bacterial species were shown to be dose-dependent. The results of the different experiments indicated that smaller NP sizes have higher antibacterial effects. M29 [Mg(OH) 2 -29] nanoparticles followed by silver (Ag) and C1 (CaCO 3 -1) showed the highest influence on bacterial growth rates, while similar ability to kill bacteria across treatment time. In addition, Gram-negative bacteria were more affected in terms of the inhibition zone and reduction of growth rates after 24 h as well as in terms of the prolonged treatment of NPs up to 36 h due to the influence of different nanoparticles. We recommend to search the chance of further using M29 and C1 in medicine and industry.