Characterization of a Pinus sylvestris thaumatin-like protein gene and determination of antimicrobial activity of the in vitro expressed protein
Thaumatin-like proteins (TLPs) are pathogenesis-related proteins, which are involved in plant defense responses to pathogen infection. Expression of the Pinus sylvestris L. TLP gene is up-regulated by methyl jasmonate treatment and inoculation with Heterobasidion annosum . A full-length Pinus taeda...
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Published in | Tree genetics & genomes Vol. 14; no. 4; pp. 1 - 13 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.08.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Thaumatin-like proteins (TLPs) are pathogenesis-related proteins, which are involved in plant defense responses to pathogen infection. Expression of the
Pinus sylvestris
L.
TLP
gene is up-regulated by methyl jasmonate treatment and inoculation with
Heterobasidion annosum
. A full-length
Pinus taeda TLP
gene sequence was used to design PCR primers for amplification of the full-length
TLP
gene from
P. sylvestris
. A putative 705-bp open reading frame of
TLP
gene was cloned into
Escherichia coli
cells, and then subcloned into the overexpression vector pET100 using BL21 Star expression bacteria. Optimization of the expression of recombinant TLP was achieved by decreasing both expression temperature and IPTG concentration. The purified 24.6-kDa TLP shows antimicrobial activity against 12 fungal species. |
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ISSN: | 1614-2942 1614-2950 |
DOI: | 10.1007/s11295-018-1274-9 |