Hirudin monitoring using the TAS ecarin clotting time in patients with heparin-induced thrombocytopenia type II

• Published in: Journal of cardiothoracic and vascular anesthesia Vol. 14; no. 3; pp. 249 - 252
• Main Authors: KOSTER, A, HANSEN, R, GRAUHAN, O, HAUSMANN, H, BAUER, M, HETZER, R, KUPPE, H, MERTZLUFFT, F
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Elsevier 01.06.2000
• Subjects: Adult; Aged; Anticoagulants - blood; Biological and medical sciences; Cardiopulmonary Bypass; Drug Monitoring; Female; Heparin - adverse effects; Hirudins - blood; Humans; Immunomodulators; Male; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Recombinant Proteins - blood; Reproducibility of Results; Thrombocytopenia - chemically induced; Whole Blood Coagulation Time; Heart; Human; Thrombocytopenia; Clotting time; Toxicity; Hemopathy; Method; Hirudin; Chemotherapy; Platelet; Treatment; Surveillance; Surgery; Glycosaminoglycan; Heparin; Recombinant protein; Reliability; Monitoring
• ISSN: 1053-0770; 1532-8422
• DOI: 10.1053/cr.2000.5862

To assess the reliability of the TAS/ecarin clotting time (ECT) for on-line monitoring of r-hirudin in cardiovascular surgery with and without cardiopulmonary bypass (CPB). Samples were spiked with r-hirudin (0 to 5 microg/mL) and calibration curves constructed. Reproducibility was evaluated by measurement of the sample five times at each concentration. The influence of variations in hematocrit, plasma factors, and platelet count on the test results was examined. Samples were obtained from patients during cardiovascular surgery with CPB (n = 8), without CPB (n = 3), and from volunteers (n = 5) and compared with the laboratory reference tests. All tests were performed in duplicate. Deutsches Herzzentrum Berlin. Five healthy volunteers and 11 patients undergoing cardiovascular surgery. None. The TAS/ECT showed linearity and reliability to an r-hirudin concentration of 5 microg/mL and was not influenced (p < 0.005) by the varying conditions of the in vitro setup. The correlation to the laboratory method was 0.74 for the CPB group and 0.87 for the non-CPB group. The TAS/ECT is a reliable assay for monitoring r-hirudin at the point of care. With this information, the use of r-hirudin during surgery or angioplasty should become more effective and safer.