Experimental Verification on the Hypothesis about the Possibility of Molecular Diagnostics of Local Tumor Spread on the Lewis Lung Carcinoma Model

• Published in: Moscow University chemistry bulletin Vol. 76; no. 4; pp. 248 - 252
• Main Authors: Bogush, T. A., Maiak, M. A., Saprykina, N. S., Basharina, A. A., Ryabinina, O. M., Grishanina, A. N., Bogush, E. A., Kamensky, A. A., Kosorukov, V. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2021; Springer Nature B.V
• Subjects: Animals; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Flow cytometry; Hypotheses; Immunofluorescence; Lungs; Markers; Proteins; Transplantation; Tumors; Lewis lung carcinoma; flow cytometry; immunofluorescent assay; beta-III tubulin (TUBB3); tissue surrounding the tumor
• ISSN: 0027-1314; 1935-0260
• DOI: 10.3103/S0027131421040039

Expression of tumor-associated protein beta-III tubulin (TUBB3) was evaluated quantitatively by immunofluorescence analysis using flow cytometry in lung tissue of intact animals and on day 11 after the Lewis lung carcinoma transplantation as well in lung metastasis tissue and in visually “normal” surrounding tissue. It was shown that the tumor is characterized by a high TUBB3 expression, while also the expression of the marker was detected in both variants of visually normal lung tissue: it was lower than in tumor tissue but significantly higher than in lung tissue of intact animals. The detection of TUBB3 outside the tumor indicates that the tissue appeared to be normal has already involved in malignancy and this supports the hypothesis that tumor-associated protein TUBB3 can be used as a molecular marker of local tumor spread.