Biological Microarray for Detection of Somatic Mutations in the Genes of Isocitrate Dehydrogenase 1 and 2

Mutations in the genes of isocitrate dehydrogenases 1 and 2 ( IDH1 and IDH2 ) are important diagnostic and prognostic markers for several tumors (chondroid tumors, brain tumors, and leukemia). The method of allele-specific hybridization on a biological microarray has been developed to evaluate mutat...

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Published inRussian journal of bioorganic chemistry Vol. 49; no. 5; pp. 1137 - 1142
Main Authors Varachev, V. O., Guskov, D. A., Shekhtman, A. P., Rogozhin, D. V., Polyakov, S. A., Zacedatelev, A. S., Chudinov, A. V., Nasedkina, T. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.10.2023
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Genes
Leukemia
Life Sciences
Mutation
Nucleotides
Organic Chemistry
Substitutes
Tumors
isocitrate dehydrogenase 1 and 2
chondroid tumors
diagnosis
mutations
glioblastoma
biological microarray
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Summary:Mutations in the genes of isocitrate dehydrogenases 1 and 2 ( IDH1 and IDH2 ) are important diagnostic and prognostic markers for several tumors (chondroid tumors, brain tumors, and leukemia). The method of allele-specific hybridization on a biological microarray has been developed to evaluate mutations in the IDH1/2 genes. This method makes it possible to detect the presence of the mutation in a tumor sample (chondroid tumors and glioblastoma) and estimate the variant of nucleotide substitution. A comparison of the methods for detection of the IDH1/2 mutation using a biological microarray and the DNA melting analysis using a TaqMan probe have shown a complete coincidence of results. The biochip method makes it possible to determine the type of nucleotide substitution without an additional sequencing step.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162023050205