Resistance of cancer cells to the TRAIL-induced apoptosis in confluent cultures

TRAIL (TNF-alpha Related Apoptosis-Inducing Ligand) is an attractive candidate for anticancer therapy. TRAIL selectively kills tumor cells, without damaging normal cells. It is known that cancer cells can acquire resistance to chemotherapeutic drugs, oxidative stress in high density culture. This ph...

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Published inBiochemistry (Moscow). Supplement series A, Membrane and cell biology Vol. 7; no. 1; pp. 29 - 36
Main Authors Fadeev, R. S., Chekanov, A. V., Dolgikh, N. V., Akatov, V. S.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 2013
Springer Nature B.V
Subjects
Apoptosis
Biochemistry
Biomedical and Life Sciences
Cancer therapies
Cell Biology
Cell culture
Drug resistance
Life Sciences
drug resistance
cancer cell lines
TRAIL/Apo2L
confluent cultures
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Summary:TRAIL (TNF-alpha Related Apoptosis-Inducing Ligand) is an attractive candidate for anticancer therapy. TRAIL selectively kills tumor cells, without damaging normal cells. It is known that cancer cells can acquire resistance to chemotherapeutic drugs, oxidative stress in high density culture. This phenomenon appears as a multi-cell resistance, cell adhesion-dependent resistance, or density-dependent resistance of tumor cells. However, it is unclear whether such resistance appears in TRAIL-induced apoptosis. We observed that the resistance to izTRAIL of all tumor cell lines used was considerably increased in confluent cultures. The increase in tumor cell resistance in dense populations is not related to their proliferative status. It was shown that the dissociation of calcium-dependent cell-cell contacts with EGTA did not suppress tumor cell resistance to izTRAIL in confluent cultures. This phenomenon of cancer cells resistance to TRAIL-induced apoptosis should be considered in the development of methods of anticancer therapy.
ISSN:1990-7478
1990-7494
DOI:10.1134/S1990747812060049