Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08

• Published in: Bioresource technology Vol. 408; p. 131228
• Main Authors: Ou, Jie, Xie, Qing, Zhong, Zi-Rou, Wang, Fei, Huang, Ming-Zhu, Fang, Zi-Xuan, Kuang, Xu-Ying, Qin, Zi-Le, Luo, Sheng-Wei
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2024
• Subjects: Acinetobacter; ammonium; aquaculture; bacteria; carbon; Degradation pathway; denitrification; Gene expression; genome; genomics; Heterotrophic nitrification and aerobic denitrification; nitrates; nitrification; nitrites; nitrogen; Nitrogen assimilation; nitrogen metabolism; total nitrogen; wastewater treatment; Gene expression; Nitrogen assimilation; Heterotrophic nitrification and aerobic denitrification; Degradation pathway
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2024.131228

[Display omitted] •Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions. A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.