Organ-specific roles for transcription factor NF-κB in reovirus-induced apoptosis and disease

Reovirus induces apoptosis in cultured cells and in vivo. In cell culture models, apoptosis is contingent upon a mechanism involving reovirus-induced activation of transcription factor NF-κB complexes containing p50 and p65/RelA subunits. To explore the in vivo role of NF-κB in this process, we test...

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Published inThe Journal of clinical investigation Vol. 115; no. 9; pp. 2341 - 2350
Main Authors O’Donnell, Sean M., Hansberger, Mark W., Connolly, Jodi L., Chappell, James D., Watson, Melissa J., Pierce, Janene M., Wetzel, J. Denise, Han, Wei, Barton, Erik S., Forrest, J. Craig, Valyi-Nagy, Tibor, Yull, Fiona E., Blackwell, Timothy S., Rottman, Jeffrey N., Sherry, Barbara, Dermody, Terence S.
Format Journal Article
LanguageEnglish
Published American Society for Clinical Investigation 01.09.2005
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Summary:Reovirus induces apoptosis in cultured cells and in vivo. In cell culture models, apoptosis is contingent upon a mechanism involving reovirus-induced activation of transcription factor NF-κB complexes containing p50 and p65/RelA subunits. To explore the in vivo role of NF-κB in this process, we tested the capacity of reovirus to induce apoptosis in mice lacking a functional nfkb1/p50 gene. The genetic defect had no apparent effect on reovirus replication in the intestine or dissemination to secondary sites of infection. In comparison to what was observed in wild-type controls, apoptosis was significantly diminished in the CNS of p50-null mice following reovirus infection. In sharp contrast, the loss of p50 was associated with massive reovirus-induced apoptosis and uncontrolled reovirus replication in the heart. Levels of IFN-β mRNA were markedly increased in the hearts of wild-type animals but not p50-null animals infected with reovirus. Treatment of p50-null mice with IFN-β substantially diminished reovirus replication and apoptosis, which suggests that IFN-β induction by NF-κB protects against reovirus-induced myocarditis. These findings reveal an organ-specific role for NF-κB in the regulation of reovirus-induced apoptosis, which modulates encephalitis and myocarditis associated with reovirus infection.
Bibliography:Address correspondence to: Terence S. Dermody, Elizabeth B. Lamb Center for Pediatric Research, D7235 Medical Center North, Vanderbilt University School of Medicine, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. Phone: (615) 343-9943; Fax: (615) 343-9723; E-mail: terry.dermody@vanderbilt.edu.
ISSN:0021-9738
DOI:10.1172/JCI22428