The screening of alpha- and beta-globin chain variants with isoelectric focusing

A simple isoelectric focusing technique is presented for the screening of alpha- and beta-globin chain variants on polyacrylamide gel in the presence of 8 M urea. No detergent was used. Variants focused at discrete pH values, depending on the charge variation (-2, -1, 0, +1 or +2) introduced by the...

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Bibliographic Details
Published inHemoglobin Vol. 6; no. 5; p. 481
Main Authors Francina, A, Baudonnet, C, Dorléac, E, Galacteros, F, Delaunay, J
Format Journal Article
LanguageEnglish
Published England 1982
Subjects
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Globins - analysis
Globins - classification
Globins - genetics
Hemoglobins, Abnormal - analysis
Hemoglobins, Abnormal - genetics
Humans
Hydrogen-Ion Concentration
Infant
Isoelectric Focusing
Male
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Summary:A simple isoelectric focusing technique is presented for the screening of alpha- and beta-globin chain variants on polyacrylamide gel in the presence of 8 M urea. No detergent was used. Variants focused at discrete pH values, depending on the charge variation (-2, -1, 0, +1 or +2) introduced by the mutation. A linear relationship was evidenced between the charge variation and the shift of the apparent isoelectric point of the variants. The practical interest of this procedure is illustrated.
ISSN:0363-0269
DOI:10.3109/03630268209083761