A multiplexer liquidchip technology for detecting single nucleotide polymorphisms from metabolism of anti-thrombotic drugs in dried blood spots on filter paper
A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent ge...
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| Published in | Acta biochimica et biophysica Sinica Vol. 46; no. 6; pp. 522 - 525 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
China
01.06.2014
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| Abstract | A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent genotypic marker for research. The elucidation of SNP information will contribute to an individual＇s suscepti bility to disease and responsiveness to drug toxicity and medical intervention [2,3]. Nowadays, a variety of techni ques have been used to perform SNP genotyping, but these techniques required whole blood as the sample. Dried blood spot （DBS） specimens require less material and are substan tially more stable （several months at room temperature） than whole blood [4]. Thus, the simplicity of sample preparation, long time storage and convenient transport make DBS to be a costeffective and suitable alternative tool for collecting blood sample. |
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| AbstractList | A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent genotypic marker for research. The elucidation of SNP information will contribute to an individual＇s suscepti bility to disease and responsiveness to drug toxicity and medical intervention [2,3]. Nowadays, a variety of techni ques have been used to perform SNP genotyping, but these techniques required whole blood as the sample. Dried blood spot （DBS） specimens require less material and are substan tially more stable （several months at room temperature） than whole blood [4]. Thus, the simplicity of sample preparation, long time storage and convenient transport make DBS to be a costeffective and suitable alternative tool for collecting blood sample. |
| Author | Zeyao Zhu Jiaying He Tao Zeng Huijuan Qin Jiasen Xu Lifen Ren-Heidenreich |
| AuthorAffiliation | SurExam Bio-Tech Co. Ltd., Guangzhou Technology Innovation Base, Science City, Guangzhou 510633, China Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24785331$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1097/01.fpc.0000184955.08453.a8 10.1056/NEJMoa0808227 10.1182/blood-2005-03-1108 10.1378/chest.119.1_suppl.8S 10.1016/0002-9343(93)90285-W 10.1016/S0140-6736(98)04474-2 10.1016/j.thromres.2006.09.007 10.1038/clpt.2008.10 10.1503/cmaj.060664 10.1093/clinchem/46.9.1498 10.1016/j.clpt.2005.11.011 10.1126/science.1105854 10.1126/science.1104635 10.1056/NEJMoa0809171 10.1016/j.cca.2007.10.001 10.1093/nar/29.1.308 |
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| Notes | 31-1940/Q Zeyao Zhu, Jiaying He, Tao Zeng, Huijuan Qin, Jiasen Xu, and Lifen Ren-Heidenreich 1.SurExam Bio-Tech Co. Ltd., Guangzhou Technology Innovation Base, Science City, Guangzhou 510633, China 2Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China ＊Correspondence address. Tel： ＋86-20-32093737; Fax： ＋86-20-32093737; E-mail： lifenren@yahoo.com A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent genotypic marker for research. The elucidation of SNP information will contribute to an individual＇s suscepti bility to disease and responsiveness to drug toxicity and medical intervention [2,3]. Nowadays, a variety of techni ques have been used to perform SNP genotyping, but these techniques required whole blood as the sample. Dried blood spot （DBS） specimens require less material and are substan tially more stable （several months at room temperature） than whole blood [4]. Thus, the simplicity of sample preparation, long time storage and convenient transport make DBS to be a costeffective and suitable alternative tool for collecting blood sample. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
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| References | Sherry ST (null) 2001; 29 Wijnen PA (null) 2008; 388 Kimura R (null) 2007; 120 Simon T (null) 2009; 360 Dunbar SA (null) 2000; 46 Sconce EA (null) 2005; 106 Takahashi H (null) 2006; 16 Wu A (null) 2007; 8 Suh JW (null) 2006; 174 Hood L (null) 2004; 306 Mega JL (null) 2009; 360 Lord PG (null) 2004; 306 Aquilante CL (null) 2006; 79 Landefeld CS (null) 1993; 95 Hirsh J (null) 2001; 119 Gage B (null) 2008; 84 Aithal GP (null) 1999; 353 |
| References_xml | – volume: 16 start-page: 101 year: 2006 ident: null publication-title: Pharmacogenet Genomics doi: 10.1097/01.fpc.0000184955.08453.a8 contributor: fullname: Takahashi H – volume: 360 start-page: 363 year: 2009 ident: null publication-title: N Engl J Med doi: 10.1056/NEJMoa0808227 contributor: fullname: Simon T – volume: 106 start-page: 2329 year: 2005 ident: null publication-title: Blood doi: 10.1182/blood-2005-03-1108 contributor: fullname: Sconce EA – volume: 119 start-page: 8 year: 2001 ident: null publication-title: Chest doi: 10.1378/chest.119.1_suppl.8S contributor: fullname: Hirsh J – volume: 95 start-page: 315 year: 1993 ident: null publication-title: Am J Med doi: 10.1016/0002-9343(93)90285-W contributor: fullname: Landefeld CS – volume: 353 start-page: 717 year: 1999 ident: null publication-title: Lancet doi: 10.1016/S0140-6736(98)04474-2 contributor: fullname: Aithal GP – volume: 120 start-page: 181 year: 2007 ident: null publication-title: Thromb Res doi: 10.1016/j.thromres.2006.09.007 contributor: fullname: Kimura R – volume: 84 start-page: 326 year: 2008 ident: null publication-title: Clin Pharmacol Ther doi: 10.1038/clpt.2008.10 contributor: fullname: Gage B – volume: 174 start-page: 1715 year: 2006 ident: null publication-title: CMAJ doi: 10.1503/cmaj.060664 contributor: fullname: Suh JW – volume: 46 start-page: 1498 year: 2000 ident: null publication-title: Clin Chem doi: 10.1093/clinchem/46.9.1498 contributor: fullname: Dunbar SA – volume: 79 start-page: 291 year: 2006 ident: null publication-title: Clin Pharmacol Ther doi: 10.1016/j.clpt.2005.11.011 contributor: fullname: Aquilante CL – volume: 306 start-page: 575 year: 2004 ident: null publication-title: Science doi: 10.1126/science.1105854 contributor: fullname: Lord PG – volume: 306 start-page: 640 year: 2004 ident: null publication-title: Science doi: 10.1126/science.1104635 contributor: fullname: Hood L – volume: 8 start-page: 865 year: 2007 ident: null publication-title: Pharmacogenomics contributor: fullname: Wu A – volume: 360 start-page: 354 year: 2009 ident: null publication-title: N Engl J Med doi: 10.1056/NEJMoa0809171 contributor: fullname: Mega JL – volume: 388 start-page: 189 year: 2008 ident: null publication-title: Clin Chim Acta doi: 10.1016/j.cca.2007.10.001 contributor: fullname: Wijnen PA – volume: 29 start-page: 308 year: 2001 ident: null publication-title: Nucleic Acids Res doi: 10.1093/nar/29.1.308 contributor: fullname: Sherry ST |
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| SubjectTerms | Antithrombins - blood Antithrombins - metabolism Base Sequence DNA Primers Humans Polymorphism, Single Nucleotide SNPs 人类基因组 单核苷酸多态性 复用器 技术检测 抗血栓 滤纸 药物代谢 |
| Title | A multiplexer liquidchip technology for detecting single nucleotide polymorphisms from metabolism of anti-thrombotic drugs in dried blood spots on filter paper |
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