A multiplexer liquidchip technology for detecting single nucleotide polymorphisms from metabolism of anti-thrombotic drugs in dried blood spots on filter paper

A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent ge...

Full description

Saved in:
Bibliographic Details
Published inActa biochimica et biophysica Sinica Vol. 46; no. 6; pp. 522 - 525
Main Authors Zhu, Zeyao, He, Jiaying, Zeng, Tao, Qin, Huijuan, Xu, Jiasen, Ren-Heidenreich, Lifen
Format Journal Article
LanguageEnglish
Published China 01.06.2014
Subjects
Antithrombins - blood
Antithrombins - metabolism
Base Sequence
DNA Primers
Humans
Polymorphism, Single Nucleotide
SNPs
人类基因组
单核苷酸多态性
复用器
技术检测
抗血栓
滤纸
药物代谢
Online AccessGet full text

Cover

More Information
Summary:A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent genotypic marker for research. The elucidation of SNP information will contribute to an individual＇s suscepti bility to disease and responsiveness to drug toxicity and medical intervention [2,3]. Nowadays, a variety of techni ques have been used to perform SNP genotyping, but these techniques required whole blood as the sample. Dried blood spot （DBS） specimens require less material and are substan tially more stable （several months at room temperature） than whole blood [4]. Thus, the simplicity of sample preparation, long time storage and convenient transport make DBS to be a costeffective and suitable alternative tool for collecting blood sample.
Bibliography:31-1940/Q
Zeyao Zhu, Jiaying He, Tao Zeng, Huijuan Qin, Jiasen Xu, and Lifen Ren-Heidenreich 1.SurExam Bio-Tech Co. Ltd., Guangzhou Technology Innovation Base, Science City, Guangzhou 510633, China 2Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China ＊Correspondence address. Tel： ＋86-20-32093737; Fax： ＋86-20-32093737; E-mail： lifenren@yahoo.com
A single nucleotide polymorphism （SNP） is the most fre quent type of variation in the genome. There are around 10 million SNPs that have been identified in the human genome [1]. Because SNPs are highly conserved throughout evolu tion and within a population, the map of SNPs serves as an excellent genotypic marker for research. The elucidation of SNP information will contribute to an individual＇s suscepti bility to disease and responsiveness to drug toxicity and medical intervention [2,3]. Nowadays, a variety of techni ques have been used to perform SNP genotyping, but these techniques required whole blood as the sample. Dried blood spot （DBS） specimens require less material and are substan tially more stable （several months at room temperature） than whole blood [4]. Thus, the simplicity of sample preparation, long time storage and convenient transport make DBS to be a costeffective and suitable alternative tool for collecting blood sample.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmu028