Overexpression of the LmHQT1 gene increases chlorogenic acid production in Lonicera macranthoides Hand-Mazz
Lonicera macranthoides Hand-Mazz contains high levels of chlorogenic acid (CGA). The CGA is synthesized via different biosynthetic pathways in various plant species, and hydroxycinnamoyl-coenzyme A quinate transferases (HQTs) are key enzymes in these routes. In this study, we isolated the LmHQT1 gen...
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Published in | Acta physiologiae plantarum Vol. 39; no. 1; pp. 1 - 10 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Lonicera macranthoides
Hand-Mazz contains high levels of chlorogenic acid (CGA). The CGA is synthesized via different biosynthetic pathways in various plant species, and hydroxycinnamoyl-coenzyme A quinate transferases (HQTs) are key enzymes in these routes. In this study, we isolated the
LmHQT1
gene, which encodes a protein of 447 amino acid residues with conserved HXXXD and DFGWG motifs. It is very closely homologous to
HQT
genes in
Lonicera japonica
(
LjHQT
),
Solanum lycopersicum
(
SlHQT
) and
Nicotiana sylvestris
(
NsHQT
). Quantitative reverse-transcription polymerase chain reaction showed that
LmHQT1
gene expression decreased following leaf senescence. The CGA contents displayed similar trends, suggesting a potential role of
LmHQT1
in CGA biosynthesis. To characterize its function,
LmHQT1
overexpressing plants were generated via
Agrobacterium
transformation methods established previously. Upregulation of
LmHQT1
in
L. macranthoides
was observed to elevate the CGA levels up to 60% in leaves. These findings indicated that
LmHQT1
was devoted to CGA biosynthesis in
L. macranthoides
. |
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ISSN: | 0137-5881 1861-1664 |
DOI: | 10.1007/s11738-016-2310-8 |