Synthesis, Characterization and Biocompatibility Studies of Gold Nanoparticles from Zingiber officinal

• Published in: BioNanoScience Vol. 7; no. 4; pp. 558 - 564
• Main Authors: Babu, R. L., Charanya, Shankar, Biradar, Santhosh K., Goornavar, Virupaxi, Jeffers, Robert, Ronurpraful, Tejaswini, Prabhu, Prathima T., Bowman, Arthur, Hall, Joseph C., Ramesh, Govindarajan T.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2017; Springer Nature B.V
• Subjects: Biocompatibility; Biological and Medical Physics; Biomaterials; Biomedical materials; Biophysics; Chloroauric acid; Circuits and Systems; Cytotoxicity; Engineering; Epithelial cells; Ginger; Gold; Nanomaterials; Nanoparticles; Nanotechnology; Oxidative stress; Reactive oxygen species; Synthesis; Toxicity; Biocompatibility; Lung epithelial cells; Reactive oxygen species; MTT assay; Gold nanoparticles; Chloroauric acid
• ISSN: 2191-1630; 2191-1649
• DOI: 10.1007/s12668-017-0427-x

Synthesis of nanomaterials through eco-friendly (green) approaches has attracted much attention due to the widespread biomedical application and environmental issues. The present study describes the green synthesis of gold nanoparticles (GNPs) using rhizome suspension of ginger ( Zingiber officinale ). The formation of GNPs was visually observed in a sample by color change from colorless to purple; further, the green synthesis process was optimized for improved production of GNPs with the suspension to chloroauric acid ratio (1:10). The synthesized GNPs were characterized by FESEM, EDX, and TEM. Ginger-based GNP synthesis gave particles of >20 nm in size. The GNPs were largely found to be spherical in shape and stable at room temperature. A live/dead cell viability assay conducted on lung epithelial cells revealed the safe nature of GNP materials up to 25 μg/ml. These results were correlated by MTT assay. It is also observed that there was no significant elevation in reactive oxygen species (ROS) level in GNP-treated lung epithelial cells (BEAS-2B). Biocompatibility studies of GNPs also revealed the negligible cytotoxicity and validate the safe nature, and no induction of oxidative stress. Collectively, these observations suggest the involvement of phytochemicals from the ginger. Future manipulation of these GNPs offers commercial benefits, particularly for biomedical applications like diagnostics and therapeutics.