Cytotoxicity and Gene Expression Studies of Curcumin and Piperine Loaded Nanoparticles on Breast Cancer Cells

• Published in: Russian journal of applied chemistry Vol. 95; no. 1; pp. 135 - 142
• Main Authors: Jafari, Duygu Aygunes, Baspinar, Yucel, Ustundas, Mehmet, Bayraktar, Oguz, Kara, Hale Guler, Sezgin, Canfeza
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2022; Springer Nature B.V
• Subjects: Anticancer properties; Bioavailability; Breast cancer; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cytotoxicity; Gene expression; Genes; Industrial Chemistry/Chemical Engineering; Nanoparticles; Toxicity; Various Technological Processes; piperine; nanoparticle; cytotoxicity; gene expression; MDA-MB-231; curcumin
• ISSN: 1070-4272; 1608-3296
• DOI: 10.1134/S1070427222010177

The aim of this study was to investigate the anticancer effects of curcumin and piperine loaded nanoparticles compared to docetaxel on the breast cancer cell line MDA-MB-231. Curcumin, exerts anti-neoplastic and anti-apoptotic activities in cancer cell lines and piperine is effective for enhancing the bioavailability of curcumin. Docetaxel is an antimicrotubule agent used for cancer treatment. The cytotoxicity and gene expression effects of curcumin-piperine carrying nanoparticles compared to docetaxel were investigated. A reliable strategy for enhancing poor solubility is encapsulation through methods like electrospray as in this study. The cytotoxic effects of nanoparticles and docetaxel on cells were determined with IC 50 values by xCELLigence real-time cell analyzer. The gene expression analysis was performed to evaluate the molecular genetic changes for cancer related genes. The genes related to cancer formation, invasion and progression such as AKT1, MYC, NOTCH1, IL6, JUN, EGFR, MAPK1, RARB, BCL2, CCND1, MAPK8, BIRC5, ESR1 revealed decreased expression in the nanoparticles group compared to docetaxel. The gene expression results of curcumin and piperine loaded nanoparticles revealed higher significant effects on MDA-MB-231 breast cancer cells in terms of cancer progression compared to docetaxel.