Spectral karyotyping (SKY) of mouse meiotic chromosomes

The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalen...

Full description

Saved in:
Bibliographic Details
Published inGenome Vol. 44; no. 2; pp. 293 - 298
Main Authors Heng, Henry HQ, Liu, Guo, Lu, Wei, Bremer, Steve, Ye, Christine J, Hughes, Mark, Moens, Peter
Format Journal Article
LanguageEnglish
Published Ottawa, Canada NRC Research Press 01.04.2001
Canadian Science Publishing NRC Research Press
Subjects
Animals
Chromosome Painting - methods
Chromosomes
Developmental stages
Genetics
Hybridization
Karyotypes
Karyotyping - methods
Male
Meiosis - genetics
Mice
Prophase - genetics
Rodents
Testis - cytology
X Chromosome - genetics
Y Chromosome - genetics
Online AccessGet full text

Cover

More Information
Summary:The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identified. This technique has the potential to recognize synaptic anomalies and chromosome-specific structural and behavioural characteristics. We confirm these potentials by the recognition of the heterologous synapsis of the X and Y chromosomes and by the variances of synaptonemal complex lengths for each of the colour-coded bivalents in eight prophase nuclei.Key words: SKY, meiosis, synaptonemal complex, multicolour, chromosome painting, spectral karyotyping, protein-SKY co-detection.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0831-2796
1480-3321
DOI:10.1139/g01-018