AMPA and NMDA receptors in dentate gyrus mediate memory for sucrose in two port discrimination task

• Published in: Hippocampus Vol. 34; no. 7; pp. 342 - 356
• Main Authors: Sagarkar, Sneha, Bhat, Nagashree, Rotti, Deepa, Subhedar, Nishikant K.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2024; Wiley Subscription Services, Inc
• Subjects: AMPA; c‐Fos; Dentate gyrus; Fos protein; Gene expression; Glutamatergic transmission; Glutamic acid receptors (ionotropic); memory; Mental task performance; N-Methyl-D-aspartic acid receptors; natural reward; NMDA; Nose; Operant conditioning; Psychotropic drugs; Sucrose; Sweet taste; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors; NMDA; natural reward; c‐Fos; AMPA; memory
• ISSN: 1050-9631; 1098-1063; 1098-1063
• DOI: 10.1002/hipo.23609

Although the phenomenon of memory formation and recall associated with the use of psychotropic drugs has been extensively studied, mechanisms underlying memories for natural reward have not been clarified. Herein, we test the hypothesis that glutamatergic receptors in the dentate gyrus play a role in memories associated with sucrose. We used pellet self‐administration protocol to generate memories in two‐port nose‐poke discrimination task using male Wistar rats. During non‐rewarded probe trial, the conditioned animals readily discriminated the active port versus inactive port and showed massive increase in mRNA expression of AMPA receptor subunit genes (gria2, gria3) as well as c‐Fos protein in the DG. Access to sweet pellet further enhanced c‐Fos expression in the DG. However, animals pre‐treated with AMPA receptor antagonist CNQX (intra‐DG), on exposure to operant chamber (no pellet), showed decreased discrimination as well as c‐Fos expression. We suggest that AMPA receptors in DG mediate recall and consolidation of memories associated with sucrose consumption. CNQX pre‐treated animals, if presented with sweet pellet on nose poke, exhibited high discrimination index coupled with increased c‐Fos expression. In these CNQX treated rats, the DI was again decreased following administration of NMDA receptor antagonist AP5. We suggest that, although AMPA receptors are blocked, the access to sweet pellet may induce surge of glutamate in the DG, which in turn may reinstate memories via activation of erstwhile silent synapses in NMDA dependant manner.