Tannic acid- and thiocarbohydrazide-mediated osmium tetroxide binding in the preparation of human leucocytes for SEM observation

Normal human leucocytes, successively treated with glutaraldehyde-tannic acid-osmium tetroxide-thiocarbohydrazide-osmium tetroxide-thiocarbohydrazide-osmium tetroxide, were prepared for scanning electron microscopy observation. These cells produced well-contrasted, non-charging scanning images compa...

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Bibliographic Details
Published inJournal of microscopy (Oxford) Vol. 132; no. Pt 2; p. 229
Main Authors Corvellec, M R, Lalague, E D, Pelletier, L, Haviernick, S, Derghazarian, C, Cousineau, G H
Format Journal Article
LanguageEnglish
Published England 01.11.1983
Subjects
Fixatives
Humans
Hydrazines
Hydrolyzable Tannins
Leukocytes - ultrastructure
Microscopy, Electron, Scanning - methods
Microvilli - ultrastructure
Osmium Tetroxide
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Summary:Normal human leucocytes, successively treated with glutaraldehyde-tannic acid-osmium tetroxide-thiocarbohydrazide-osmium tetroxide-thiocarbohydrazide-osmium tetroxide, were prepared for scanning electron microscopy observation. These cells produced well-contrasted, non-charging scanning images compatible with metal-evaporated material. Further, the mononuclear and polymorphonuclear cells resisted shrinkage during dehydration and critical point drying, thus allowing much improved images at high magnification than those covered with evaporated metal. In all cases at least a second thiocarbohydrazide-osmium tetroxide treatment could not be avoided.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.1983.tb04277.x