Transcriptomic Profiles of MV4-11 and Kasumi 1 Acute Myeloid Leukemia Cell Lines Modulated by Epigenetic Modifiers Trichostatin A and 5-Azacytidine

• Published in: International journal of hematology- oncology and stem cell research Vol. 14; no. 1; pp. 72 - 92
• Main Authors: Asmaa, Mat Jusoh Siti, Al-Jamal, Hamid Ali, Hussein, Abdul Rahim, Yahaya, Badrul Hisham, Hassan, Roslin, Hussain, Faezahtul Arbaeyah, Shamsuddin, Shaharum, Johan, Muhammad Farid
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences, Hematology-Oncology and Stem Cell Transplantation Research Center 01.01.2020; Tehran University of Medical Sciences
• Subjects: 5-Azacytidine; Acute myeloid leukemia; Epigenetics; Gene expression; Histone deacetylase inhibitor; Original; Gene expression; Acute myeloid leukemia; 5-Azacytidine; Epigenetics Histone deacetylase inhibitors
• ISSN: 2008-3009; 2008-2207; 2008-2207
• DOI: 10.18502/ijhoscr.v14i1.2362

Acute myeloid leukemia (AML) is the most common form of acute leukemias in adults which is clinically and molecularly heterogeneous. Several risk and genetic factors have been widely investigated to characterize AML. However, the concomitant epigenetic factors in controlling the gene expression lead to AML transformation was not fully understood. This study was aimed to identify epigenetically regulated genes in AML cell lines induced by epigenetic modulating agents, Trichostatin A (TSA) and 5-Azacytidine (5-Aza). MV4-11 and Kasumi 1 were treated with TSA and/or 5-Aza at IC concentration. Gene expression profiling by microarray was utilized using SurePrint G3 Human Gene Expression v3. Gene ontology and KEGG pathway annotations were analyzed by DAVID bioinformatics software using EASE enrichment score. mRNA expression of the differentially expressed genes were verified by quantitative real time PCR. Gene expression analysis revealed a significant changes in the expression of 24,822, 15,720, 15,654 genes in MV4-11 and 12,598, 8828, 18,026 genes in Kasumi 1, in response to TSA, 5-Aza and combination treatments, respectively, compared to non-treated ( <0.05). 7 genes ( , , , , , and ) and 4 genes ( , , and ) shown to be predominantly expressed in MV4-11 and Kasumi 1, respectively (EASE<0.1). The analysis also revealed phagosome pathway commonly activated in both cell lines. Our data showed a distinct optimal biological characteristic and pathway in different types of leukemic cell lines. These finding may help in the identification of cell-specific epigenetic biomarker in the pathogenesis of AML.