Characterization of cultivable intestinal microbiota in Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) and determination of its cellulolytic activity

• Published in: Archives of insect biochemistry and physiology Vol. 110; no. 2; pp. e21881 - n/a
• Main Authors: Calumby, Rodrigo J. N., Almeida, Lara M., Barros, Yasmin N., Segura, Wilson D., Barbosa, Valcilaine T., Silva, Antonio T., Dornelas, Camila B., Alvino, Valter, Grillo, Luciano A. M.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2022
• Subjects: Alcaligenes; Arthrobacter; Bacteria; Biological control; Cellulose; Citrobacter; Cocos nucifera; Dilution; Flow chambers; Fungi; Insects; intestinal microbiota; Intestinal microflora; Intestine; Ionization; Laminar flow; MALDI‐TOF; Mass spectrometry; Mass spectroscopy; Microbiota; Microorganisms; Pest control; Pests; Rhynchophorus palmarum; Saline solutions; Substrates; intestinal microbiota; Rhynchophorus palmarum; MALDI-TOF; Cocos nucifera; cellulose
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.21881

Rhynchophorus palmarum Linnaeus is an agricultural pest that affects various palm crops, including coconut (Cocos nucifera) plantations which are prominent in the economy of Northeastern Brazil. Characterization of the intestinal microbiota of R. palmarum, as well as elucidation of aspects related to the biochemistry and physiology of the insect's digestion, is essential for intervention in specific metabolic processes as a form of pest control. Thus, this study aimed to characterize the intestinal microbiota of R. palmarum and investigate its ability to degrade cellulosic substrates, to explore new biological control measures. Intestinal dissection of eight adult R. palmarum insects was performed in a laminar flow chamber, and the intestines were homogenized in sterile phosphate‐buffered saline solution. Subsequently, serial dilution aliquots of these solutions were spread on nutritive agar plates for the isolation of bacteria and fungi. The microorganisms were identified by matrix‐assisted laser desorption/ionization with a time‐of‐flight mass spectrometry and evaluated for their ability to degrade cellulose. Fourteen bacterial genera (Acinetobacter, Alcaligenes, Arthrobacter, Bacillus, Citrobacter, Enterococcus, Kerstersia, Lactococcus, Micrococcus, Proteus, Providencia, Pseudomonas, Serratia, and Staphylococcus) and two fungal genera (Candida and Saccharomyces)—assigned to the Firmicutes, Actinobacteria, Proteobacteria, and Ascomycota phyla—were identified. The cellulolytic activity was exhibited by six bacterial and one fungal species; of these, Bacillus cereus demonstrated the highest enzyme synthesis (enzymatic index = 4.6). This is the first study characterizing the R. palmarum intestinal microbiota, opening new perspectives for the development of strategies for the biological control of this insect. The cultivable intestinal microbiota of adult insects of Rhynchophorus palmarum was characterized using matrix‐assisted laser desorption/ionization with a time‐of‐flight mass spectrometry (MALDI‐TOF/MS). Fourteen bacterial and two fungal genera were identified. The Firmicutes and Proteobacteria phyla were identified here as being quite prevalent among bacteria, and Enterococcus faecalis, as being the most prevalent bacterial species. The cellulolytic activity was exhibited by both bacterial (six) and fungal (one) species, indicating that cellulose‐degrading microorganisms colonize the R. palmarum gut. highlights tdiversity. a is