The Kinetics of Allergen-induced Eotaxin Level in Nasal Lavage Fluid . Its Key Role in Eosinophil Recruitment in Nasal Mucosa

• Published in: American journal of respiratory and critical care medicine Vol. 164; no. 4; pp. 575 - 579
• Main Authors: TERADA, NOBUHISA, HAMANO, NANAKO, KIM, WOO JEONG, HIRAI, KOICHI, NAKAJIMA, TOSHIHARU, YAMADA, HIROKAZU, KAWASAKI, HIROSHI, YAMASHITA, TETSUJI, KISHI, HIROHISA, NOMURA, TOMOHIRO, NUMATA, TSUTOMU, YOSHIE, OSAMU, KONNO, AKIYOSHI
• Format: Journal Article
• Language: English
• Published: New York, NY Am Thoracic Soc 15.08.2001; American Lung Association
• Subjects: Allergens - adverse effects; Allergic diseases; Antibodies, Monoclonal - pharmacology; Antibodies, Monoclonal - therapeutic use; Biological and medical sciences; Case-Control Studies; Chemokine CCL11; Chemokine CCL5 - analysis; Chemokine CCL5 - immunology; Chemokines, CC; Chemotactic Factors, Eosinophil - analysis; Chemotactic Factors, Eosinophil - immunology; Chemotaxis, Leukocyte - drug effects; Chemotaxis, Leukocyte - immunology; Cytokines - analysis; Cytokines - antagonists & inhibitors; Cytokines - immunology; Cytokines - metabolism; Endothelium, Vascular - drug effects; Endothelium, Vascular - immunology; Endothelium, Vascular - ultrastructure; Eosinophils - drug effects; Eosinophils - immunology; Humans; Immunopathology; Inflammation; Leukocyte Count; Medical sciences; Nasal Lavage Fluid - chemistry; Nasal Lavage Fluid - immunology; Nasal Mucosa - chemistry; Nasal Mucosa - cytology; Nasal Mucosa - drug effects; Nasal Mucosa - immunology; Nasal Provocation Tests; Receptors, CCR3; Receptors, Chemokine - antagonists & inhibitors; Respiratory and ent allergic diseases; Rhinitis, Allergic, Seasonal - blood; Rhinitis, Allergic, Seasonal - drug therapy; Rhinitis, Allergic, Seasonal - immunology; Rhinitis, Allergic, Seasonal - pathology; Human; Immunopathology; Allergy; Biological fluid; Nose disease; Rhinitis; Pathogenesis; Washing; Eosinophil; Recruitment; Nose; ENT disease; Evolution; Kinetics; Allergen; Provocation test
• ISSN: 1073-449X; 1535-4970
• DOI: 10.1164/ajrccm.164.4.2009046

Eotaxin (CCL11) is a potent eosinophil chemoattractant belonging to the C-C chemokine. To evaluate the role of eotaxin in eosinophilic inflammation in nasal mucosa, we investigated the levels of eosinophil chemoattractants in nasal lavage fluids obtained after antigen challenge, compared with eosinophil counts and eosinophil protein X (EPX) levels. In subjects with allergic rhinitis, allergen challenge led to parallel increases in eosinophil counts, levels of EPX, and eotaxin concentrations in nasal lavage fluid. The levels of eotaxin in lavage samples showed strong correlation with lavage levels of eosinophil counts and EPX. Normal subjects had few, if any, eosinophils and EPX as well as the measured parameters in their nasal lavage fluids before and after antigen challenge. In our experiments of eosinophil endothelial transmigration (TEM) assay using the nasal microvascular endothelial cells, eotaxin showed the most potent effect among various eosinophil chemoattractants. In addition, treatment of eosinophils with anti-CCR-3 mAb significantly blocked eosinophil TEM induced by homogenate of nasal mucosa. These results indicate that eotaxin has an important role in eosinophil-dependent inflammation in nasal mucosa and suggest that blocking eotaxin or CCR-3 might be useful for new therapeutic tools of allergic rhinitis.