In vitro plant regeneration from organogenic callus of Curcuma kwangsiensis Lindl. (Zingiberaceae)

A novel protocol for callus-mediated shoot regeneration was established for an important medicinal and ornamental plant native to South China, Curcuma kwangsiensis , using shoot base sections excised from seedlings in vitro as explant sources. The frequency of callus formation reached 91% for explan...

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Bibliographic Details
Published inPlant growth regulation Vol. 64; no. 2; pp. 141 - 145
Main Authors Zhang, Shijun, Liu, Nian, Sheng, Aiwu, Ma, Guohua, Wu, Guojiang
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.06.2011
Springer Nature B.V
Subjects
Agriculture
Biomedical and Life Sciences
Growth regulators
Life Sciences
Original Paper
Plant Anatomy/Development
Plant growth
Plant Physiology
Plant Sciences
Seedlings
Plant regeneration
Callus
Rhizome
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Summary:A novel protocol for callus-mediated shoot regeneration was established for an important medicinal and ornamental plant native to South China, Curcuma kwangsiensis , using shoot base sections excised from seedlings in vitro as explant sources. The frequency of callus formation reached 91% for explants cultured on MS medium containing 1.4 μM TDZ, 4.4 μM BA and 2.3 μM 2,4-D. 8.2 shoots per callus was achieved on MS medium supplemented with 1.4 μM TDZ, 17.8 μM BA and 2.7 μM NAA. Single shoots transferred into MS medium free of plant growth regulator rooted well. Regenerated plants acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse conditions.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-010-9548-8