Changes in fatty acids contents and growth characteristics in transformed oilseed rape (Brassica napus)

• Published in: Biologia plantarum Vol. 35; no. 3
• Main Authors: Dusbabkova, J, Necasek, J. (Akademie Ved Ceske Republiky, Ceske Budejovice (Czech Republic). Ustav Molekularni Biologie Rostlin), Bohacova, I, Bezecna, L, Konradova, M
• Format: Journal Article
• Language: English
• Published: 01.09.1993
• Subjects: ACIDE GRAS; ACIDOS GRASOS; ADN; AGROBACTERIUM RHIZOGENES; BRASSICA NAPUS; CRECIMIENTO; CROISSANCE; CULTIVOS DE PRIMAVERA; CULTURE DE PRINTEMPS; DESCENDANCE; GRAINE; HABITOS DE CRECIMIENTO; PLASMIDE; PLASMIDIOS; PORT DE LA PLANTE; PROGENIE; SEMILLA; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/BF02928508

Spring oilseed rape Brassica napus ssp. oleifera cv. HM-81 was transformed with TL-DNA of the Ri plasmid of the agropine strain Agrobacterium rhizogenes 15834. Selfed progenies (R2 and R3 generations) were studied for changes in values of growth characteristics and fatty acids contents. Transformants are homozygous for TL-DNA. Both generations of transformants differed significantly from the nontransformed control plants in reduced length, lower number of pods per plant, lower total mass of seeds and the higher number of branches. The contents of palmitic, linoleic and linolenic acids were significantly higher in transformants when compared with the control. On the contrary, the contents of both stearic and oleic acids were in most of transformants significantly lower. Only traces of erucic acids (less than 0,05 %) were found, both in transformed and nontransformed plants.