Importance of the high-molecular-mass isoform of adiponectin in improved insulin sensitivity with rosiglitazone treatment in HIV disease

• Published in: Clinical science (1979) Vol. 115; no. 5-6; pp. 197 - 202
• Main Authors: QURASHI, Saima, MYNARCIK, Dennis C, MCNURLAN, Margaret A, AHN, Hongshik, FERRIS, Robert, GELATO, Marie C
• Format: Journal Article
• Language: English
• Published: London Portland Press 01.09.2008
• Subjects: Adiponectin - blood; Adiponectin - physiology; Adult; Biological and medical sciences; Blood Glucose - metabolism; CD4 Lymphocyte Count; Female; General aspects; HIV Infections - blood; HIV Infections - drug therapy; HIV Infections - virology; Human viral diseases; Humans; Hypoglycemic Agents - therapeutic use; Infectious diseases; Insulin - blood; Insulin Resistance; Liver - metabolism; Male; Medical sciences; Middle Aged; Molecular Weight; Protein Isoforms - blood; Protein Isoforms - physiology; Thiazolidinediones - therapeutic use; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viral Load; Endocrinopathy; Molecular form; Adiponectin; Hypoglycemic agent; Molecular mass; High; Immunopathology; thiazolidinedione; Molar mass; Retroviridae; Metabolic diseases; AIDS; Thiazolidinedione derivatives; Immune deficiency; Lentivirus; Insulin; Infection; Virus; Medicine; Target tissue resistance; Treatment; HIV; Viral disease; Rosiglitazone; Insulin resistance; Human immunodeficiency virus
• ISSN: 0143-5221; 1470-8736
• DOI: 10.1042/CS20070387

The present study was designed to investigate the relationship of isoforms of adiponectin to insulin sensitivity in subjects with HIV-associated insulin resistance in response to treatment with the thiazolidinedione, rosiglitazone. The two isoforms of adiponectin, HMW (high-molecular-mass) and LMW (low-molecular-mass), were separated by sucrose-gradient-density centrifugation. The amount of adiponectin in gradient fractions was determined by ELISA. Peripheral insulin sensitivity (Rd) was determined with hyperinsulinaemic-euglycaemic clamp, whereas hepatic sensitivity [HOMA (Homoeostasis Model Assessment) %S] was based on basal glucose and insulin values. Treatment with rosiglitazone for 3 months resulted in a significant improvement in the index of hepatic insulin sensitivity (86.4+/-15% compared with 139+/-23; P=0.007) as well as peripheral insulin sensitivity (4.04+/-0.23 compared with 6.17+/-0.66 mg of glucose/kg of lean body mass per min; P<0.001). Improvement in HOMA was associated with increased levels of HMW adiponectin (r=0.541, P=0.045), but not LMW adiponectin. The present study suggests that the HMW isoform of adiponectin is important in the regulation of rosiglitazone-mediated improvement in insulin sensitivity in individuals with HIV-associated insulin resistance, particularly in the liver.