Biorefinery-centric ethanol and oleochemical production employing Yarrowia lipolytica and Pichia farinosa

• Published in: Bioresource technology Vol. 394; p. 130243
• Main Authors: Manasa, Sravya, Tharak, Athmakuri, Venkata Mohan, S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2024
• Subjects: Biodiesel; Circular economy; FAD12; Green Carbon; Single cell oil; Single cell protein; Single cell protein; FAD12; Circular economy; Green Carbon; Biodiesel; Single cell oil
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.130243

[Display omitted] •Food waste and effluent yield valuable oleochemicals and alcohol synthesis.•YL exhibits highest lipid production in synthetic carbon systems.•FAD2 and ADH expression are high in synthetic carbon than waste carbon source.•Both YL and PF produced high protein content during growth phase.•A biorefinery framework was established to synthesize the chemicals from food-waste. The research examined the capabilities of Yarrowia lipolytica (YL) and Pichia farinosa (PF) in converting sugars to ethanol and oleochemicals. Lipid, ethanol, protein yield and gene-expressions were analysed at different substrate concentrations (3 to 30 g/L) with glucose, food waste, and fermentation-effluent. Optimal results were obtained at 20 g/L using both synthetic carbon with 4.6 % of total lipid yield. Lauric and Caprylic acid dominance was noted in total lipid fractions. Protein accumulation (6 g/L) was observed in glucose system (20 g/L) indicating yeast strains potential as single-cell proteins (SCP). Fatty-acid desaturase (FAD12) and alcohol dehydrogenase (ADH) expressions were higher at optimum condition of YL (1.15 × 10−1, 3.8 × 10−2) and PF (5.8 × 10−2, 3.8 × 10−2) respectively. Maximum carbon reduction of 87 % depicted at best condition, aligning with metabolic yield. These findings highlights promising role of yeast as biorefinery biocatalyst.