Concentrations of estrone, estradiol, and estrone sulfate and evaluation of sulfatase and aromatase activities in pre- and postmenopausal breast cancer patients

• Published in: The journal of clinical endocrinology and metabolism Vol. 81; no. 4; pp. 1460 - 1464
• Main Authors: PASQUALINI, J. R, CHETRITE, G, BLACKER, C, FEINSTEIN, M.-C, DELALONDE, L, TALBI, M, MALOCHE, C
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.04.1996
• Subjects: Adult; Aged; Aromatase - metabolism; Biological and medical sciences; Breast - chemistry; Breast - enzymology; Breast Neoplasms - blood; Breast Neoplasms - chemistry; Breast Neoplasms - enzymology; Estradiol - analysis; Estradiol - blood; Estrogens - analysis; Estrogens - blood; Estrone - analogs & derivatives; Estrone - analysis; Estrone - blood; Female; Gynecology. Andrology. Obstetrics; Humans; Mammary gland diseases; Medical sciences; Menstrual Cycle; Middle Aged; Postmenopause; Premenopause; Reference Values; Sensitivity and Specificity; Sulfatases - metabolism; Tumors; Human; Carcinoma; Premenopause; Enzyme; Pathogenesis; Tumoral tissue; Estrogen; Esterases; Malignant tumor; Estrogen synthase; Sulfates; Estradiol; Ovarian hormone; Assay; Mammary gland diseases; Arylsulfatase; Postmenopause; Hydrolases; Estrone; Serum; Mammary gland; Sex steroid hormone; Sulfuric ester hydrolases
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jc.81.4.1460

This report concerns the evaluation of various estrogens, estrone (El), estradiol (E2), and estrone sulfate (E1S), as well as E1S-sulfatase and aromatase activities in pre- and postmenopausal women with breast cancer. The levels (in picomoles per g; mean +/- SEM) of the various estrogens in the breast tissue from premenopausal patients (n = 11) are: El, 1.4 +/- 0.5; E2, 1.2 +/- 0.6; and E1S, 1.2 +/- 0.3. In postmenopausal patients (n = 23), the values are, respectively, 1.0 +/- 0.4, 1.4 +/- 0.7, and 3.3 +/- 1.9. These concentrations of estrogens in the tumors of postmenopausal patients are significantly higher than those found in plasma. The activity of E1S-sulfatase in both pre- and postmenopausal patients was 50-200 times higher than that of aromatase. E1S-sulfatase and aromatase activities are significantly higher in post-menopausal than in cycling patients. It is concluded that despite the low levels of circulating estrogens in postmenopausal patients, the tissue concentrations of these steroids are several-fold higher than those in plasma, suggesting tumor accumulation of these estrogens. The physiopathology and clinical significance of these high levels of the various estrogens (E1, E2, and E1S) as well as sulfatase and aromatase activities in postmenopausal patients with breast cancer is yet to be explored.