In vitro measurement of superoxide dismutase-like nanozyme activity: a comparative study

• Published in: Analyst (London) Vol. 146; no. 6; pp. 1872 - 1879
• Main Authors: Liu, Yufeng, Zhang, Yihong, Liu, Quanyi, Wang, Quan, Lin, Anqi, Luo, Jie, Du, Yan, Lin, Ying-Wu, Wei, Hui
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 21.03.2021
• Subjects: Biological effects; Cerium oxides; Comparative studies; Electron paramagnetic resonance; Electron Spin Resonance Spectroscopy; In vitro methods and tests; Oxidation-Reduction; Pigments; Riboflavin; Sensitivity analysis; Superoxide dismutase; Superoxide Dismutase - metabolism; Water
• ISSN: 0003-2654; 1364-5528; 1364-5528
• DOI: 10.1039/D0AN02164D

Analyzing the SOD-like activity of nanozymes in vitro is of great importance for identifying new nanozymes and predicting their potential biological effects in vivo . However, false negative or positive results occasionally occur due to the mismatch between the detection methods and nanozymes. Here, five typical SOD-like nanozymes, including CeO 2 , Mn 3 O 4 , Prussian blue (PB), PCN222-Mn, and Pt NPs, have been used to evaluate the sensitivity and accuracy of several commonly used in vitro detection methods. By systematically analyzing the detection results, several precautions have been taken. (1) The hydroethidine (HE) probe could be disturbed by the nanozyme with oxidative ability. (2) The nitro blue tetrazolium (NBT) probe has a moderate sensitivity due to the poor water solubility of its reduced product. (3) The water-soluble tetrazolium salt (WST)-8 probe has a higher sensitivity than both NBT and iodonitrotetrazolium chloride (INT). (4) The detection system using the irradiation of riboflavin to produce ˙O 2 − might be interfered by the nanozyme with photosensibility. (5) Both the quality of DMPO and incubation time are important factors for electron paramagnetic resonance (EPR) measurement. This study will be useful for choosing more suitable in vitro detection methods of SOD-like activity for nanozymes in the future.