High-Na+ low-K+ UW cold storage solution reduces reperfusion injuries of the rat liver graft

• Published in: Transplant international Vol. 11; no. 3; pp. 223 - 230
• Main Authors: BEN ABDENNEBI, H, STEGHENS, J.-P, MARGONARI, J, RAMELLA-VIRIEUX, S, BARBIEUX, A, BOILLOT, O
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing 20.05.1998
• Subjects: Adenosine; Alanine Transaminase - metabolism; Allopurinol; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Aspartate Aminotransferases - metabolism; Biological and medical sciences; Clinical death. Palliative care. Organ gift and preservation; Creatine Kinase - metabolism; Cryopreservation; Glutathione; Indocyanine Green - pharmacokinetics; Insulin; Isoenzymes; Liver - enzymology; Liver Circulation; Liver Transplantation - methods; Male; Medical sciences; Microcirculation; Organ Preservation Solutions; Potassium; Raffinose; Rats; Rats, Wistar; Reperfusion Injury - prevention & control; Sodium; Rat; Potassium ion; Liver; Rodentia; Cold storage; Isolated organ; Vertebrata; Mammalia; Organ preservation; Reperfusion; Animal; Sodium ion; Lesion; Technique; Solution
• ISSN: 0934-0874; 1432-2277
• DOI: 10.1007/s001470050132

The isolated perfused rat liver model was used to assess graft viability after 24 h of cold preservation. Two solutions were compared for liver preservation: Belzer's original UW solution (high-K+ UW) and a solution containing the same components but with inverted concentrations of sodium and potassium (high-Na+ UW). During the 120 min of normothermic reperfusion, livers preserved in the high-Na+ UW solution released lower levels of creatine kinase-BB isoenzyme, transaminases (ALT and AST), and potassium than those preserved in the high-K+ UW solution. Bile flow and biliary excretion of indocyanine green increased when livers were preserved in the high-Na+ UW solution. We found no statistical differences for oxygen consumption and tissue ATP concentration. The results of this study support the concept that a high-Na+ UW solution is a more effective means of preserving rat livers, at least after 24 h of cold-storage and 120 min of reperfusion in the isolated perfused model, than the original high-K+ UW solution. Liver preservation in the high-Na+ UW solution reduces damage to sinusoidal endothelial and hepatocellular cells. The use of an extracellular-like Belzer cold storage solution eliminates potassium-related problems in cold preservation and subsequent normothermic reperfusion while keeping all the qualities of the original UW solution.