Improved detection and comparative sizing of human chromosomal telomeres in situ

Telomeric length dynamics are thought to play an important role both in the processes of cellular aging and cancer progression. We have revised the primed in situ (PRINS) labeling technique to allow an estimation of the relative length of individual telomeres. We illustrate the applicability of the...

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Bibliographic Details
Published inChromosoma Vol. 107; no. 3; pp. 198 - 203
Main Authors Krejcí, K, Koch, J
Format Journal Article
LanguageEnglish
Published Austria Springer Nature B.V 01.06.1998
Subjects
Adolescent
Adult
Aged
Aged, 80 and over
Bone marrow
Chromosomes - genetics
Chromosomes, Human, Pair 11 - genetics
DNA Primers - genetics
Female
Fluorescent Dyes
Humans
In Situ Hybridization, Fluorescence - methods
Indoles
Male
Repetitive Sequences, Nucleic Acid - genetics
Staining and Labeling - methods
Telomere - genetics
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Summary:Telomeric length dynamics are thought to play an important role both in the processes of cellular aging and cancer progression. We have revised the primed in situ (PRINS) labeling technique to allow an estimation of the relative length of individual telomeres. We illustrate the applicability of the approach by demonstrating different telomeric sizes not only between blood lymphocytes from a young and an old donor, but also among bone marrow cells from hematological cancer patients. In the latter case we found general variations in telomeric sizes as well as individual telomeric variations that would have escaped detection by other methods. An interesting finding was the selective expansion of a single telomere within a specific subset of cells.
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ISSN:0009-5915
1432-0886
DOI:10.1007/s004120050297