Performance, HSP70 expression, antioxidant enzymes, oxidative DNA damage biomarkers, metabolic hormones, and intestinal microbiota of broiler chickens fed mistletoe leaf powder supplemented diets under tropical high ambient temperatures

• Published in: Journal of thermal biology Vol. 121; p. 103861
• Main Authors: Oloruntola, Olugbenga David, Ayodele, Simeon Olugbenga, Oloruntola, Deborah Adebukola, Olarotimi, Olumuyiwa Joseph, Falowo, Andrew Bamidele, Akinduro, Victor Olabisi, Adeniji, Olufemi Emmanuel, Adu, Olufemi Adesanya, Gbore, Francis Ayodeji
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2024
• Subjects: Birds; Heat stress; Natural antioxidants; Oxidative stress; Phytogenic supplements; Oxidative stress; Birds; Phytogenic supplements; Heat stress; Natural antioxidants
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/j.jtherbio.2024.103861

The study investigated the impact of Mistletoe Leaf Powder (MLP) supplementation on some parameters in heat-stressed broiler chickens. The standard baseline diets, comprising four different formulations, were provided during the starter and finisher stages. Chickens were randomly assigned to the 4 dietary groups: a negative control (CON) with no supplementation, a positive control (VTC) with 200 mg/kg vitamin C, and 2 experimental treatment groups with 2500 mg/kg (MLP2) and 5000 mg/kg (MLP5) MLP supplementation. The Body Weight Gain (BWG) in MLP2 and MLP5 treatment groups was comparable (P > 0.05) to those in VTC, while the CON group exhibited significantly (P < 0.05) lower BWG. Feed consumption was significantly (P < 0.05) lower broiler chickens in the CON group compared to those VTC, MLP2, and MLP5. Heat shock protein 70 (HSP70) levels were lower in broiler chickens belonging to VTC, MLP2, and MLP5 groups compared to those in CON, and MLP2 showed no difference (P > 0.05) from MLP5 and VTC. Serum glutathione peroxidase and catalase concentrations were higher (P < 0.05) in birds belonging to MLP5, MLP2, and VTC groups compared to CON. The 8-hydroxy-2′-deoxyguanosine concentration was lower (P < 0.05) in birds of VTC, MLP2, and MLP5 compared to the CON, with VTC showing the least concentration. Serum insulin levels were higher (P < 0.05) in MLP5 compared to those in CON, while serum triiodothyronine and leptin concentrations were lower (P < 0.05) in CON compared to birds in VTC, MLP2, and MLP5. Microbiota analysis revealed that the Coliform bacteria population was higher (P < 0.05) in birds belonging to CON compared to those in VTC, MLP2, and MLP5 groups, whereas lactic acid-producing bacteria were significantly (P < 0.05) lower in birds of CON and highest in MLP2 and MLP5 groups. In conclusion, dietary supplementation of MLP at 5000 mg/kg enhanced performance, oxidative status, influenced metabolic hormones, and gut microbiota in broiler chickens raised under high ambient temperature. •Mistletoe leaf powder boosts body weight gain in heat-stressed broiler chickens.•Mistletoe leaf powder increases feed consumption compared to the negative control.•Mistletoe leaf powder modulates HSP70 levels, reducing cellular stress responses.•Mistletoe leaf powder enhances serum antioxidants, mitigating oxidative stress.•Mistletoe leaf powder influences metabolic hormones and gut microbiota balance.