Design and fabrication of an angle-scanning based platform for the construction of surface plasmon resonance biosensor

• Published in: Optics and lasers in engineering Vol. 78; pp. 1 - 7
• Main Authors: Hu, Jiandong, Cao, Baiqiong, Wang, Shun, Li, Jianwei, Wei, Wensong, Zhao, Yuanyuan, Hu, Xinran, Zhu, Juanhua, Jiang, Min, Sun, Xiaohui, Chen, Ruipeng, Ma, Liuzheng
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.03.2016
• Subjects: An area CCD array; Angle-scanning; Laser line generator; Optic SPR biosensing platform; Angle-scanning; Optic SPR biosensing platform; Laser line generator; An area CCD array
• ISSN: 0143-8166; 1873-0302
• DOI: 10.1016/j.optlaseng.2015.09.004

A sensing system for an angle-scanning optical surface-plasmon-resonance (SPR) based biosensor has been designed with a laser line generator in which a P polarizer is embedded to utilize as an excitation source for producing the surface plasmon wave. In this system, the emitting beam from the laser line generator is controlled to realize the angle-scanning using a variable speed direct current (DC) motor. The light beam reflected from the prism deposited with a 50nm Au film is then captured using the area CCD array which was controlled by a personal computer (PC) via a universal serial bus (USB) interface. The photoelectric signals from the high speed digital camera (an area CCD array) were converted by a 16 bit A/D converter before it transferred to the PC. One of the advantages of this SPR biosensing platform is greatly demonstrated by the label-free and real-time bio-molecular analysis without moving the area CCD array by following the laser line generator. It also could provide a low-cost surface plasmon resonance platform to improve the detection range in the measurement of bioanalytes. The SPR curve displayed on the PC screen promptly is formed by the effective data from the image on the area CCD array and the sensing responses of the platform to bulk refractive indices were calibrated using various concentrations of ethanol solution. These ethanol concentrations indicated with volumetric fraction of 5%, 10%, 15%, 20%, and 25%, respectively, were experimented to validate the performance of the angle-scanning optic SPR biosensing platform. As a result, the SPR sensor was capable to detect a change in the refractive index of the ethanol solution with the relative high linearity at the correlation coefficient of 0.9842. This greatly enhanced detection range is obtained from the position relationship between the laser line generator and the right-angle prism to allow direct quantification of the samples over a wide range of concentrations. •We report an angle-scanning optical surface-plasmon-resonance (SPR) based biosensor designed with a laser line generator.•The laser beam is only driven to scan automatically on the surface of the biosensor while the area CCD camera is fixed without moving.•It is capable to detect a change in the refractive index of the ethanol solution with the relative high linearity at the correlation coefficient of 0.9842.•The expanded detection range could be achieved effectively.