A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

• Published in: Analyst (London) Vol. 143; no. 22; pp. 5512 - 5519
• Main Authors: Wu, Yongquan, Shi, Aiping, Li, Yuanyan, Zeng, Hong, Chen, Xiaoyong, Wu, Jie, Fan, Xiaolin
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 05.11.2018
• Subjects: Animals; Cells (biology); Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - toxicity; Fluorescent indicators; Humans; In vivo methods and tests; Inflammation - metabolism; Limit of Detection; MCF-7 Cells; Mice; Peroxynitrous Acid - analysis; Phosphinic Acids - chemical synthesis; Phosphinic Acids - chemistry; Phosphinic Acids - toxicity; Physiology; RAW 264.7 Cells; Selectivity; Spectrometry, Fluorescence - methods; Xanthenes - chemical synthesis; Xanthenes - chemistry; Xanthenes - toxicity
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c8an01107a

Peroxynitrite (ONOO-) plays important roles in the regulation of many physiological and pathological processes, and an increase in its levels is related to numerous diseases. Thus, accurate detection of ONOO- in physiological conditions is imperative for elucidating its functions. However, studies on high signal-to-noise-ratio (SNR) fluorescence imaging of ONOO-in vivo for its detection are currently lacking. Thus, a novel NIR xanthene fluorescence probe (NOF2) for the endogenous detection of ONOO- is designed and synthesized. The fluorescence of the NOF2 probe is pre-quenched by the hydroxyl protection group of diphenyl phosphinate. Additionally, the NOF2 probe exhibits good selectivity and sensitivity for ONOO- with a low detection limit of 0.40 μM. Importantly, the NOF2 probe displays good performances for the detection of endogenous ONOO- not only in living cells but also in a mouse inflammation model. This demonstrates its great potential for applications involving the detection of ONOO- both in vitro and in vivo to explore the roles of ONOO- in different physiological systems.