Improving NanoCluster Beacon performance by blocking the unlabeled NC probes

While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 55; no. 4; pp. 462 - 465
Main Authors Chen, Yu-An, Vu, Huong T, Liu, Yen-Liang, Chen, Yuan-I, Nguyen, Trung Duc, Kuo, Yu-An, Hong, Soonwoo, Chen, Yin-An, Carnahan, Savannah, Petty, Jeffrey T, Yeh, Hsin-Chih
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 03.01.2019
Subjects
Blocking
Deoxyribonucleic acid
DNA
Gene sequencing
Nanoclusters
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Summary:While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc08291j