Comparative Analysis of the Probiotic Features of Lysinibacillus and Enterobacter Strains Isolated from Gut Tract of Triploid Cyprinid Fish

• Published in: Current microbiology Vol. 82; no. 2; p. 91
• Main Authors: Fang, Zi-Xuan, Kuang, Xu-Ying, Li, Yao-Hui, Yu, Ruo-Xing, Wang, Fei, Luo, Sheng-Wei
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.02.2025
• Subjects: Animals; Aquaculture; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Biofilms; Biofilms - growth & development; Comparative analysis; Cyprinidae; Cyprinidae - microbiology; digestive system; Enterobacter; Enterobacter - classification; Enterobacter - genetics; Enterobacter - isolation & purification; Enterobacter - physiology; farmed fish; Fish; Gastrointestinal Microbiome; Gastrointestinal Tract - microbiology; Gene sequencing; genome; Genomic analysis; Hydrophobicity; Lymphoid tissue; Lysinibacillus; Microbiota; Microorganisms; Mucosal immunity; Mucosal-associated lymphoid tissue; Probiotics; Strains (organisms); Triploidy; Whole Genome Sequencing
• ISSN: 0343-8651; 1432-0991; 1432-0991
• DOI: 10.1007/s00284-025-04074-8

Gut mucosal immunity of teleost is mainly governed by mucosa-associated lymphoid tissues (MALT) and indigenous microbiota on mucosal surfaces of gut tract, which can confer protection against pathogenic invasion. However, the probiotic features of bacterial isolates from gut tract of triploid cyprinid fish (TCF) were largely unclear. In this study, Lysinibacillus and Enterobacter strains were isolated for probiotic identification. Whole genome sequencing (WGS) analysis indicated that Lysinibacillus and Enterobacter isolates possessed a variety of functional genes associated with probiotic features. Biofilm-forming activity (BFA) were one of the most important probiotic features, which can enable probiotic strains to communicate with indigenous microbiota by forming sessile community and then confer protection against stressors and invading pathogens. In this study, Lysinibacillus and Enterobacter isolates displayed high levels of BFA, hydrophobicity as well as aggregating potentials. Moreover, supernatants of probiotic isolates not only decreased pathogenic BFA and growth activity, but also showed high decomposing activity to macronutrients. These results indicated that probiotic isolates from gut tract of TCFs may pose protective roles in health of farmed fish.