Anti-oxidative and anti-inflammatory effects of Rosa Mosqueta oil supplementation in rat liver ischemia-reperfusion

• Published in: Food & function Vol. 9; no. 9; pp. 4847 - 4857
• Main Authors: Dossi, Camila G, González-Mañán, Daniel, Romero, Nalda, Silva, David, Videla, Luis A, Tapia, Gladys S
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 19.09.2018
• Subjects: alpha-Linolenic Acid - metabolism; alpha-Linolenic Acid - therapeutic use; alpha-Tocopherol - metabolism; alpha-Tocopherol - therapeutic use; Animals; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use; Antioxidants - therapeutic use; Carbonyl compounds; Carbonyls; Cytokines; Dietary Supplements; Docosahexaenoic Acids - metabolism; Edible oils; Eicosapentaenoic Acid - metabolism; Fatty acids; gamma-Tocopherol - metabolism; gamma-Tocopherol - therapeutic use; Gene expression; Gene Expression Regulation; Heme; Heme oxygenase (decyclizing); Histology; IL-1β; Inflammation; Injury prevention; Ischemia; Linolenic acid; Liver; Liver - blood supply; Liver - immunology; Liver - metabolism; Liver - pathology; Liver transplantation; Male; NADP; NF-E2-Related Factor 2 - antagonists & inhibitors; NF-E2-Related Factor 2 - metabolism; NF-κB protein; Oil; Oils, Volatile - therapeutic use; Oxidative Stress; Oxygenase; Parameters; Preconditioning; Pretreatment; Proteins; Quinone oxidoreductase; Random Allocation; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - immunology; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; Rodents; Rosa - chemistry; Seeds - chemistry; Signal Transduction; Supplements; Therapeutic applications; Thiobarbituric acid; Tocopherols; Transaminases; Transplantation; Tumor necrosis factor; Tumor necrosis factor-TNF; Weaning
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/c8fo00969d

Ischemia-reperfusion (IR) is a deleterious condition associated with liver transplantation or resection that involves pro-oxidant and pro-inflammatory mechanisms. Considering that Rosa Mosqueta (RM) oil composition is rich in protective components such as α-linolenic acid (ALA) and tocopherols, we studied the effects of RM oil supplementation given prior to an IR protocol. Male Sprague-Dawley rats receiving RM oil (0.4 mL d-1) for 21 days were subjected to 1 h of ischemia followed by 20 h reperfusion. Parameters of liver injury (serum transaminases, histology), oxidative stress [liver contents of protein carbonyls, thiobarbituric acid reactants, Nrf2 activity and its target mRNA expression of heme oxygenase-1 (HO-1) and NADPH-quinone oxidoreductase-1 (NQO-1)] and inflammation [nuclear factor-κB (NF-κB) and its target mRNA expression of tumor necrosis factor-α (TNF-α) and interleukine-1β (IL-1β)] were studied. RM oil increased liver ALA and its derived EPA and DHA fatty acids' contents, with enhancement in those of α- and γ-tocopherols. IR induced inflammatory liver injury, with enhancement in serum transaminases, oxidative stress-related parameters with reduced Nrf2 signaling, and higher pro-inflammatory cytokines, indexes that were attenuated or abrogated by RM oil pretreatment. It is concluded that RM oil supplementation represents a novel non-invasive preconditioning strategy against liver injury induced by IR that has potential clinical applications in metabolic stress conditions.