Association of miR-502-binding site single nucleotide polymorphism in the 3＇-untranslated region of SET8 and TP53 codon 72 polymorphism with non-small cell lung cancer in Chinese population

• Published in: Acta biochimica et biophysica Sinica Vol. 46; no. 2; pp. 149 - 154
• Main Authors: Yang, Shaodi, Guo, Haiyan, Wei, Benjie, Zhu, Shengcui, Cai, Yanlin, Jiang, Pei, Tang, Jianxin
• Format: Journal Article
• Language: English
• Published: China 01.02.2014
• Subjects: 3' Untranslated Regions - genetics; Adult; Asian Continental Ancestry Group - genetics; Carcinoma, Non-Small-Cell Lung - genetics; Case-Control Studies; Female; Gene Frequency; Genetic Predisposition to Disease; Histone-Lysine N-Methyltransferase - genetics; Humans; Lung Neoplasms - genetics; Male; MicroRNAs - genetics; Middle Aged; Polymorphism, Single Nucleotide; Risk; Tumor Suppressor Protein p53 - genetics; 中国人群; 关联; 单核苷酸多态性; 密码子; 结合位点; 限制性片段长度多态性; 非小细胞肺癌; 非翻译区; SET8; non-small cell lung cancer; polymorphism; tp53
• ISSN: 1672-9145; 1745-7270; 1745-7270
• DOI: 10.1093/abbs/gmt138

The objective of this study was to identify whether the miR-502-binding site single nucleotide polymorphism （SNP） in the 3＇-untranslated region （3＇-UTR） of set domain-containing protein 8 （SET8） and the tumor protein p53 （TP53） codon 72 polymorphism were associated with the risk for non-small cell lung cancer （NSCLC）, either in- dependently or jointly, among Chinese people from south- ern Han. The genotypes of SET8 and TP53 codon 72 polymorphisms of peripheral blood DNA were detected using polymerase chain reaction-restriction fragment length polymorphism and direct DNA sequencing in a case-control study on 164 NSCLC cases and 199 controls. The SET8 TT （odds ratio, OR = 2.173, 95% confidence interval, CI = 1.0454.517） or TP53 GG （OR = 2.579, 95% CI = 1.366-4.870） genotype was associated with an increased risk of NSCLC by comparing with the SET8 CC or TP53 CC genotype, respectively. Similar results were obtained in SET8 recessive model （OR = 2.074, 95% CI = 1.019-4.221, P〈 0.05）, and the dominant and recessive model of TP53 codon 72 were performed, respectively （OR = 1.809, 95% CI = 1.159-2.825, P〈 0.05; OR = 1.933, 95% CI = 1.096-3.409, P 〈 0.05）. In addition, inter- action between the SET8 and TP53 polymorphisms increased the risk of NSCLC in a multiply manner, with the OR being 3.032 （95%CI = 1.580-5.816） for subjects carrying both SET8 TT and TP53 GG genotypes. Therefore, the miR-502-binding site SNP in the 3＇-UTR of SET8 and the TP53 codon 72 polymorphism may he markers of genetic susceptibility to NSCLC in Chinese population, and there is a possible gene-gene interaction in the incidence of NSCLC.