Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis
This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer,...
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| Published in | Chinese science bulletin Vol. 53; no. 19; pp. 2953 - 2957 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Heidelberg
SP Science in China Press
01.10.2008
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| Abstract | This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes. |
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| AbstractList | This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes. This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked olyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes. |
| Author | ZHOU Jin XU JianDong XIE Yao QU Feng DENG YuLin GENG LiNa |
| AuthorAffiliation | School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
| Author_xml | – sequence: 1 givenname: Jin surname: Zhou fullname: Zhou, Jin organization: School of Life Science and Technology, Beijing Institute of Technology – sequence: 2 givenname: JianDong surname: Xu fullname: Xu, JianDong organization: School of Life Science and Technology, Beijing Institute of Technology – sequence: 3 givenname: Yao surname: Xie fullname: Xie, Yao organization: School of Life Science and Technology, Beijing Institute of Technology – sequence: 4 givenname: Feng surname: Qu fullname: Qu, Feng organization: School of Life Science and Technology, Beijing Institute of Technology – sequence: 5 givenname: YuLin surname: Deng fullname: Deng, YuLin organization: School of Life Science and Technology, Beijing Institute of Technology – sequence: 6 givenname: LiNa surname: Geng fullname: Geng, LiNa email: lngeng@eyou.com organization: School of Life Science and Technology, Beijing Institute of Technology |
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| Keywords | protein separation semi-crosslinked polyacrylamide capillary sieving electrophoresis |
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| Notes | Q51 11-1785/N capillary sieving electrophoresis, semi-crosslinked polyacrylamide, protein separation |
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| Title | Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis |
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